20.500.12556/RUL-134676 Transcriptomics of receptive endometrium in women with sonographic features of adenomyosis Background: Women with uterine adenomyosis seeking assisted reproduction have been associated with compromised endometrial receptivity to embryo implantation. To understand the mechanisms involved in this process, we aimed to compare endometrial transcriptome profles during the window of implantation (WOI) between women with and without adenomyosis. Methods: We obtained endometrial biopsies LH-timed to the WOI from women with sonographic features of adenomyosis (n=10) and controls (n=10). Isolated RNA samples were subjected to RNA sequencing (RNA-seq) by the Illumina NovaSeq 6000 platform and endometrial receptivity classifcation with a molecular tool for menstrual cycle phase dating (beREADY®, CCHT). The program language R and Bioconductor packages were applied to analyse RNA-seq data in the setting of the result of accurate endometrial dating. To suggest robust candidate pathways, the identifed diferentially expressed genes (DEGs) associated with the adenomyosis group in the receptive phase were further integrated with 151, 173 and 42 extracted genes from published studies that were related to endometrial receptivity in healthy uterus, endometriosis and adenomyosis, respectively. Enrichment analyses were performed using Cytoscape ClueGO and CluePedia apps. Results: Out of 20 endometrial samples, 2 were dated to the early receptive phase, 13 to the receptive phase and 5 to the late receptive phase. Comparison of the transcriptomics data from all 20 samples provided 909 DEGs (p<0.05; nonsignifcant after adjusted p value) in the adenomyosis group but only 4 enriched pathways (Bonferroni p value < 0.05). The analysis of 13 samples only dated to the receptive phase provided suggestive 382 DEGs (p<0.05; nonsignifcant after adjusted p value) in the adenomyosis group, leading to 33 enriched pathways (Bonferroni p value <0.05). These included pathways were already associated with endometrial biology, such as “Expression of interferon (IFN)-induced genes” and “Response to IFN-alpha”. Data integration revealed pathways indicating a unique efect of adenomyosis on endometrial molecular organization (e.g., “Expression of IFN-induced genes”) and its interference with endometrial receptivity establishment (e.g., “Extracellular matrix organization” and “Tumour necrosis factor production”). Conclusions: Accurate endometrial dating and RNA-seq analysis resulted in the identifcation of altered response to IFN signalling as the most promising candidate of impaired uterine receptivity in adenomyosis. adenomyosis assisted reproductive techniques (ART) data integration endometrial receptivity enrichment pathway analysis omics approaches RNA-seq systems biology transcriptomics window of implantation medicina adenomioza genetika true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-25 13:11:55 2022-01-25 13:11:56 2022-09-13 03:56:29 0000-00-00 00:00:00 2022 0 0 16 str. art. 2 Vol. 20 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616:575 1477-7827 10.1186/s12958-021-00871-5 91852035 RAZ_Prasnikar_Erika_2022.pdf RAZ_Prasnikar_Erika_2022.pdf 1 4AE9651B70E7E05A5ACB5553943F2A98 e5163694395e46431943cade67dbb3064be74af24cf7024b1fdee555652b6939 cca20313-7dd9-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153238 https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00871-5 1 c9857fa5-7dd7-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153234 Biotehniška fakulteta 0 0 0