20.500.12556/RUL-134670 Determination of neonicotinoid pesticides in propolis with liquid chromatography coupled to tandem mass spectrometry In this study, a method was developed for the determination of five neonicotinoid pesticides (acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam) in propolis. Two sample preparation methods were tested: solid-phase extraction and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. The identities of analytes were confirmed using liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the selected reaction monitoring mode. Solid-phase extraction resulted in cleaner extracts; therefore, the SPE-LC-MS/MS method was validated according to the SANTE protocol in triplicate at two spiking levels (10 ng/g and 50 ng/g). The average recoveries of analytes ranged from 61% to 101%, except for clothianidin (10–20%). The LOD ranged from 0.2 ng/g to 4.4 ng/g, whereas the LOQ was in the range of 0.8 ng/g–14.7 ng/g. In order to compensate for the matrix effect, matrix-matched calibration was used. Good accuracy (relative error: 1.9–10.4%) and good linearity (R$^2$ > 0.991) were obtained for all compounds. The optimised method was applied to 30 samples: 18 raw propolis and 12 ethanol tinctures. Acetamiprid, imidacloprid, and thiacloprid were detectable in seven samples but were still below the LOQ. This study is the first to report the determination of several neonicotinoid residues in propolis. neonicotinoids propolis SPE QuEChERS LC-MS/MS neonikotinoidi propolis SPE QuEChERS LC-MS/MS true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-25 10:22:12 2022-01-25 10:22:13 2022-09-13 03:56:26 0000-00-00 00:00:00 2020 0 0 12 str. iss. 24, art. 5870 Vol. 25 Dec. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544.5:543.51 1420-3049 10.3390/molecules25245870 42430723 RAZ_Tomsic_Rok_2020.pdf RAZ_Tomsic_Rok_2020.pdf 1 DFCE572781EFE6E948CC73E77421D99D f80fa1ad7d6f71f5f18f221a9fc2f0cfa144a6c22eedfc48c14ba71ecd610655 6a76ea5e-7dc0-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153224 https://www.mdpi.com/1420-3049/25/24/5870 0 15075773-7dc0-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153223 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0