20.500.12556/RUL-134659 Scanning electron microscopy protocol for studying anatomy of highly degraded waterlogged archaeological wood Waterlogged archaeological wood (WAW), approximately 4500 years old, from the prehistoric pile-dwelling settlement at Ljubljansko barje, Slovenia, was examined by scanning electron microscopy (SEM). We propose a simplified protocol for sample preparation and the SEM technique for the study of highly degraded WAW of Quercus, Faxinus, Acer, Salix and Populus, representing taxa with different wood properties. We present the advantages of the proposed technique for wood identification, the observation of various anatomical features and for the study of cell wall degradation. SEM, equipped with energy-dispersive X-ray spectroscopy (EDX), allowed us to detect significant amounts of Fe, S and Ca with different appearances, amounts and distributions in the wood of the studied taxa. In the case of Populus, an increased amount of Si was also detected. The applied SEM protocol allowed characterisation of the anatomy of the highly degraded WAW while reducing the time required for sample preparation and examination under the microscope, as well as extending the lifetime of the SEM components (e.g., tungsten filament), compared to the situation when we analyse wood samples with a greater volume. waterlogged archaeological wood scanning electron microscopy wood anatomy wood preservation EDX protocol vrstična elektronska mikroskopija anatomija lesa zaščita lesa protokol true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-24 14:28:26 2022-01-24 14:28:32 2022-09-13 03:56:22 0000-00-00 00:00:00 2022 0 0 17 str. iss. 2, art. 161 Vol. 13 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 2022-01-19 0000-00-00 630*8 1999-4907 10.3390/f13020161 94546947 RAZ_Balzano_Angela_2022.pdf RAZ_Balzano_Angela_2022.pdf 1 989DFECCC7C468FCEA93CCFEC7419F0B 5392648620028e1774d4a5f2e669874368b8908bf6a4fbd22b19a4b1011d0fa0 18164365-7d1a-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153207 https://www.mdpi.com/1999-4907/13/2/161 1 5377447a-7d19-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153206 Biotehniška fakulteta 0 0 0