20.500.12556/RUL-134651 Improving fungal decay resistance of less durable sapwood by impregnation with Scots pine knotwood and black locust heartwood hydrophilic extractives with antifungal or antioxidant properties Research Highlights: The antifungal assay confirmed that knotwood extractives of Scots pine inhibit the growth of wood decay fungi. Heartwood extracts of black locust were found to be much stronger free radical scavengers than the extracts of Scots pine. The extracts were deposited in the lumina and on the wall surface of cells in the impregnated sapwood. Impregnation of the sapwood blocks with Scots pine and black locust extracts reduced the fungal decay of wood. Objectives: Hydrophilic extracts of Scots pine knotwood and black locust heartwood were chemically analyzed, tested for antifungal and antioxidant properties and used for impregnation of beech and Scots pine sapwood. Materials and Methods: Scots pine knotwood and black locust heartwood were extracted, and obtained hydrophilic extractives were chemically analyzed. Extracts were analyzed for antifungal properties with the in vitro well-diffusion method. The free radical scavenging activity of wood extracts was measured colorimetrically. The retention of the extracts in the impregnated sapwood blocks was evaluated with microscopy and gravimetry. A decay test was performed with the mini block test. Results: Almost half of both Scots pine knotwood and black locust heartwood hydrophilic extracts obtained were described by phenolic compounds. The extracts were deposited in the lumina of cells and on the cell wall surface. Extractives of Scots pine knotwood had good inhibitory properties against white- and brown-rot fungi. On the other hand, extractives of black locust heartwood were found to be good radical scavengers, better than knotwood extractives of Scots pine. The extracts of Scots pine knotwood and black locust reduced the fungal decay of the tested sapwood blocks. Conclusions: The results of this research show that the less-valued knotwood of Scots pine and heartwood of black locust are a potential source of antifungal and antioxidant agents for bio-based wood preservatives. Pinus sylvestris Robinia pseudoacacia hydrophilic extractives wood impregnation fungal growth inhibition radical scavenging activity decay resistance Pinus sylvestris Robinia pseudoacacia hidrofilni ekstraktivi impregnacija lesa inhibicija rasti gliv lovljenje prostih radikalov odpornost proti razkroju true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-24 08:56:13 2022-01-24 08:56:20 2022-09-13 03:56:20 0000-00-00 00:00:00 2020 0 0 23 str. iss. 9, art. 1024 Vol. 11 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*813 1999-4907 10.3390/f11091024 29712643 RAZ_Vek_Viljem_2020.pdf RAZ_Vek_Viljem_2020.pdf 1 AA01C3B6F7E8AFDA3857464ECEDCD176 de2e8ec8372ca1f7ea955c7af5d378dfbd448979caa0ce96bacce8db205beabe 2a666192-7ceb-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153190 https://www.mdpi.com/1999-4907/11/9/1024 0 0bb069bc-7ceb-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153189 Biotehniška fakulteta 0 0 0