20.500.12556/RUL-134622 Biopotential of underutilized Rosaceae inflorescences LC-DAD-MS phytochemical profiles associated with antioxidant, antidiabetic, anti-inflammatory and antiproliferative activity in vitro The aim of this work was to assess the biopotential of the young inflorescence tissues of Prunus, Malus and Chaenomeles in order to evaluate the possibility of their application in the food industry, and to provide a polyphenolic fingerprint for their quality control. The contents of different bioactive compounds and their antioxidant capacities were spectrophotometrically measured, the main phenolic compounds were identified and quantified using LC-DAD-MS, the antidiabetic potential was determined using α-amylase and α-glucosidase inhibition assays, the antiinflammatory potential was determined using a 5-lipoxygenase inhibition assay, and the cytotoxicity was determined by MTT assay. Using one-way ANOVA, principal component analysis, hierarchical clustering and Pearson’s correlation coefficient, the relations between the samples, and between the samples and the measured parameters, were revealed. In total, 77 compounds were identified. The concentration of sugars was low in M. purpurea, at 1.56 ± 0.08 mg/g DW. The most effective sample in the inhibition of antidiabetic enzymes and anti-inflammatory 5-lipoxygenase was C. japonica. The inhibition of α-glucosidase was strongly positively correlated with the total and condensed tannins, procyanidin dimers and procyanidin tetramer, and was very strongly correlated with chlorogenic acid. In α-amylase inhibition, C. japonica and P. serrulata ‘Kiku Shidare Zakura’ were equally efficient to the standard inhibitor, maltose. The most effective in the growth and proliferation inhibition of HepG2, HCT116 and HaCaT cells was P. avium. The results suggest Prunus, Malus and Chaenomeles inflorescences as functional food ingredients. 5-lipoxygenase α-amylase α-glucosidase Chaenomeles Malus Prunus metabolomics colorectal carcinoma hepatocellular carcinoma keratinocytes Prunus Malus Chaenomeles Rosaceae metabolomika encimi alfa amilaza alfa glukozidaza karcinom debelega črevesa in danke true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-21 11:26:12 2022-01-21 11:26:19 2022-09-13 03:56:10 0000-00-00 00:00:00 2022 0 0 19 str. iss. 3, art. 271 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.1/..7 2223-7747 10.3390/plants11030271 94414339 plants-11-00271-v2.pdf plants-11-00271-v2.pdf 1 A28FE4743CD43D4FE38069A7FF368877 c94f0fd908d31548c679b03a9caaa499e6fc6946e1548e469d37275a2a961e92 b40f3c89-e574-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=157074 https://www.mdpi.com/2223-7747/11/3/271 1 6193a81e-7aa4-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153144 Biotehniška fakulteta 0 0 0