20.500.12556/RUL-134462 Silver fir (Abies alba L.) polyphenolic extract shows beneficial influence on chondrogenesis in vitro under normal and inflammatory conditions Several plant polyphenols have been shown to reduce osteoarthritis symptoms due to their antioxidant, anti-inflammatory and immunomodulatory properties. We investigated the effects of two different polyphenolic extracts (Belinal, Pycnogenol) and two different polyphenols (resveratrol, quercetin) on the chondrogenic potential of bone-derived mesenchymal stem/stromal cells (MSCs) from healthy donors and patients with osteoarthritis. Our main aim was to determine whether Belinal, a commercially available polyphenolic extract from silver fir (Abies alba L.) branches, has comparable chondrogenic potential with the other tested extract and the polyphenols under inflammatory and non-inflammatory conditions. In our study, Belinal promoted significantly greater chondrogenesis compared to the untreated (p = 0.0289) and resveratrol-treated (p = 0.0468) MSCs from patients with hip osteoarthritis under non-inflammatory conditions. Under inflammatory conditions, chondrogenesis was significantly enhanced for MSCs treated with Belinal compared to the control (p = 0.0483). The other extract and the polyphenols did not show any significant effects on chondrogenesis under non-inflammatory or inflammatory conditions. None of the tested extracts and polyphenols showed significant effects on chondrogenesis in healthy donors, under either non-inflammatory or inflammatory conditions. Our data show that Belinal can boost the chondrogenesis of MSCs derived from patients with osteoarthritis, under both non-inflammatory and inflammatory conditions. mesenchymal stem cells mesenchymal stromal cells polyphenols chondrogenesis inflammation subchondral bone osteoarthritis polifenoli stromalne celice hondrogeneza vnetje osteoartritis mezenhimske matične celice true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-17 09:30:52 2022-01-17 09:30:53 2022-09-13 03:55:19 0000-00-00 00:00:00 2020 0 0 14 str. iss. 20, art. 4616 Vol. 25 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.72-002 1420-3049 10.3390/molecules25204616 32142339 RAZ_Sirse_Mateja_2020.pdf RAZ_Sirse_Mateja_2020.pdf 1 02AE6B2532131DE311BFF931ECCECCD5 a9f1fa7556c8ccb12b19adc409642cdd536c3ccbc1fc280652ff07381946da18 e51e658e-776f-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152928 https://www.mdpi.com/1420-3049/25/20/4616 1 98c4397c-776f-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152927 Fakulteta za farmacijo 0 0 0