20.500.12556/RUL-134373 Critical impact of different conserved endoplasmic retention mofits and dopamine receptor interacting proteins (DRIPs) on intracellular localization and trafficking of the D$_2$ dopamine receptor (D$_2$-R) isoforms The type 2 dopamine receptor D$_2$ (D$_2$-R), member of the G protein-coupled receptor (GPCR) superfamily, exists in two isoforms, short (D$_{2S}$-R) and long (D$_{2L}$-R). They differ by an additional 29 amino acids (AA) in the third cytoplasmic loop (ICL3) of the D$_{2L}$-R. These isoforms differ in their intracellular localization and trafficking functionality, as D$_{2L}$-R possesses a larger intracellular pool, mostly in the endoplasmic reticulum (ER). This review focuses on the evolutionarily conserved motifs in the ICL3 of the D$_2$-R and proteins interacting with the ICL3 of both isoforms, specifically with the 29 AA insert. These motifs might be involved in D$_2$-R exit from the ER and have an impact on cell-surface and intracellular localization and, therefore, also play a role in the function of dopamine receptor signaling, ligand binding and possible homo/heterodimerization. Our recent bioinformatic data on potential new interaction partners for the ICL3 of D$_2$-Rs are also presented. Both are highly relevant, and have clinical impacts on the pathophysiology of several diseases such as Parkinson’s disease, schizophrenia, Tourette’s syndrome, Huntington’s disease, manic depression, and others, as they are connected to a variety of essential motifs and differences in communication with interaction partners. D$_2$ dopamine receptor intracellular trafficking endoplasmic reticulum retention motifs ICL3 interacting partners true false true Angleški jezik Ni določen Članek v reviji 2022-01-12 08:59:35 2022-01-12 08:59:41 2023-03-20 15:23:38 0000-00-00 00:00:00 2020 0 0 18 str. iss. 10, art. 1355 Vol. 10 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2218-273X 10.3390/biom10101355 29478403 RAZ_Blagotinsek_Cokan_Kaja_2020.pdf RAZ_Blagotinsek_Cokan_Kaja_2020.pdf 1 1CDE1EF5503A9276561FC6937E29E6AB c2d994dda9790068483463619a9268d8772efefde6a8f17fbcffb02acd1155c4 e4306929-737e-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152790 https://www.mdpi.com/2218-273X/10/10/1355 0 6b1b34d3-737d-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152786 Veterinarska fakulteta 0 0 0