20.500.12556/RUL-134368 Alteration of the phenylpropanoid pathway by watercore disorder in apple (Malus x domestica) Watercore is a physiological disorder of apple expressed as fluid deposition in intercellular spaces. We analysed the watercore prone cultivar 'Fuji' to establish a link between enzymatic response and fluid accumulation in the fruit. Individual phenolic content, selected enzyme activities of the phenylpropanoid pathway, sugars and organic acids were quantified in watercore affected flesh and peel. In addition, transcriptome sequencing was performed to obtain a first insight into molecular mechanisms underlying the physiological disorder. Sampled material included: peel (HP) and flesh from healthy fruit (H), peel from watercore affected fruit (WP), healthy flesh from fruit with watercore (HW) and watercore flesh (WW) from fruit with watercore. A sorbitol increase in watercore affected apple was observed not only in the flesh but also in the peel. Moreover, two phenolic groups (hydroxycinnamic acids and flavonols) were significantly affected by watercore and their content was higher in WP in comparison to HP. Dihydrochalcone content was the highest in WW flesh. This was supported by RNA-seq data, which indicated a generally increased expression of genes from the early flavonoid pathway, sorbitol metabolism and polyphenol oxidases. Among the phenylpropanoid pathway enzymes analysed, phloretin 2′-O-glycosyltransferase was the only one exhibiting altered activity. dihydrochalcones enzyme activity gene expression flavonoid pathway phenolic compounds physiological disorder jabolka dihidrohalkoni aktivnost encimov izražanje genov sintezna pot flavonoidov fenolne spojine fiziološke motnje Malus x domestica true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-11 12:32:26 2022-01-11 12:32:29 2022-09-13 03:54:45 0000-00-00 00:00:00 2021 0 0 9 str. art. 110438 Vol. 289 17 Nov. 2021 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-07-31 2023-07-31 634 1879-1018 72043779 RAZ_Cebulj_Anka_2021.pdf RAZ_Cebulj_Anka_2021.pdf 1 FF0ACE4D9254EBE4EC99B4D0C0B00A32 d04d29c3375b0f39eb05154f711f6f6d6ec48261d121f132c7cce8f3d3a74c01 42818e05-7525-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152888 https://www.sciencedirect.com/science/article/pii/S0304423821005458?dgcid=author 1 fbb953fc-72d1-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152778 Biotehniška fakulteta 0 0 0