20.500.12556/RUL-134274 Tetracycline induces the formation of biofilm of bacteria from different phases of wastewater treatment The study monitored the effect of tetracycline on bacterial biofilm formation and compared biofilm formation by resistant bacterial strains in different phases of the wastewater treatment process in wastewater treatment plant (WWTP). The crystal violet staining method was used to evaluate the biofilm formation. Biofilm-related bacterial properties were characterized by hydrophobicity, autoaggregation and motility tests. The relative abundance of tetracycline resistance genes (tetW, tetM, tetO, tetA and tetB) in wastewaters were subsequently quantified using qPCR. The results show that the isolates from the nitrification tank produce biofilm with up to 10 times greater intensity relative to the isolates from the sedimentation tank. In isolates of Aeromonas sp. from the nitrification tank, increased biofilm production in the occurrence of tetracycline from a concentration of 0.03125 µg/mL was observed. The tetW gene showed the highest relative abundance out of all the tested genes. From the sampling points, its abundance was the highest in the sedimentation tank of the WWTP. Based on these results, it can be assumed that resistant bacteria are able to form a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation. WWTPs thus represent a reservoir of antibiotic resistance genes and contribute to the spread of resistance in the natural environment. biofilm tetracycline tetracycline resistance bacteria tetracycline resistance genes wastewater treatment plant tetraciklin biofilm true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-04 11:46:20 2022-01-04 11:46:20 2022-09-13 03:54:19 0000-00-00 00:00:00 2020 0 0 20 str. iss. 8, art. 989 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.2 2227-9717 10.3390/pr8080989 25693955 RAZ_Stachurova_Tereza_2020.pdf RAZ_Stachurova_Tereza_2020.pdf 1 34FC6C8AAA5F6E9BB795582223A2B2DC 3cae8a4c8173dbfead36483b2426e77c74ed82baae3410221f5ac8330698d428 a016411d-6d4b-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152646 https://www.mdpi.com/2227-9717/8/8/989 1 63ce0578-6d4b-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152645 Biotehniška fakulteta 0 0 0