20.500.12556/RUL-134235 Molecular study of selected taxonomically critical taxa of the genus Iris L. from the broader Alpine-Dinaric area Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., I. sibirica subsp. erirrhiza, I. x croatica and I. x rotschildii). The first molecular data on I. adriatica revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers. Iridaceae Europe chloroplast DNA microsatellites phylogeny taxonomy Iridaceae mikrosatelitski markerji filogenija molekularna biologija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-03 10:54:04 2022-01-03 10:54:05 2022-09-13 03:54:05 0000-00-00 00:00:00 2020 0 0 16 str. iss. 9, art. 1229 Vol. 9 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 2223-7747 10.3390/plants9091229 29832451 RAZ_Weber_Tim_2020.pdf RAZ_Weber_Tim_2020.pdf 1 C220668B133BC96D27930E5208B9C189 fd3f93f8be5e04cfd9a2a75093be62322681820b4ef075a12540f34fcedd1253 23810250-6c7b-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152583 https://www.mdpi.com/2223-7747/9/9/1229 1 ed217211-6c7a-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152582 Biotehniška fakulteta 0 0 0