20.500.12556/RUL-133795 The ComX quorum sensing peptide of Bacillus subtilis affects biofilm formation negatively and sporulation positively Quorum sensing (QS) is often required for the formation of bacterial biofilms and is a popular target of biofilm control strategies. Previous studies implicate the ComQXPA quorum sensing system of Bacillus subtilis as a promoter of biofilm formation. Here, we report that ComX signaling peptide deficient mutants form thicker and more robust pellicle biofilms that contain chains of cells. We confirm that ComX positively affects the transcriptional activity of the P$_{epsA}$ promoter, which controls the synthesis of the major matrix polysaccharide. In contrast, ComX negatively controls the P$_{tapA}$ promoter, which drives the production of TasA, a fibrous matrix protein. Overall, the biomass of the mutant biofilm lacking ComX accumulates more monosaccharide and protein content than the wild type. We conclude that this QS phenotype might be due to extended investment into growth rather than spore development. Consistent with this, the ComX deficient mutant shows a delayed activation of the pre-spore specific promoter, P$_{spoIIQ}$, and a delayed, more synchronous commitment to sporulation. We conclude that ComX mediated early commitment to sporulation of the wild type slows down biofilm formation and modulates the coexistence of multiple biological states during the early stages of biofilm development. biofilm pellicle quorum sporulation bacillus signal communication heterogeneity matrix surfactin mikrobne združbe Bacillus subtilis zaznavanje kvoruma biofilm sporulacija surfaktin true false true Angleški jezik Slovenski jezik Članek v reviji 2021-12-15 12:06:10 2021-12-15 12:06:11 2022-09-13 03:52:41 0000-00-00 00:00:00 2020 0 0 20 str. iss. 8, art. 1131 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:579.852.11 2076-2607 10.3390/microorganisms8081131 24045827 RAZ_Spacapan_Mihael_2020.pdf RAZ_Spacapan_Mihael_2020.pdf 1 A4CE01637BABE6CAE6D5A5A4B3254682 61a9a7a77d81e86d87571561330724e263b722aa0202e8adf4dc9441bbece07f 1496fb6c-5d97-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152135 https://www.mdpi.com/2076-2607/8/8/1131 1 e430313c-5d96-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152134 Biotehniška fakulteta 0 0 0