20.500.12556/RUL-133736 Characterization of plasma-derived small extracellular vesicles indicates ongoing endothelial and platelet activation in patients with thrombotic antiphospholipid syndrome Antiphospholipid syndrome (APS) is a systemic autoimmune disease, characterized by thrombosis, obstetric complications and the presence of antiphospholipid antibodies (aPL), which drive endothelial injury and thrombophilia. Extracellular vesicles (EVs) have been implicated in endothelial and thrombotic pathologies. Here, we characterized the quantity, cellular origin and the surface expression of biologically active molecules in small EVs (sEVs) isolated from the plasma of thrombotic APS patients (n = 14), aPL-negative patients with idiopathic thrombosis (aPL-neg IT, n = 5) and healthy blood donors (HBD, n = 7). Nanoparticle tracking analysis showed similar sEV sizes (110–170 nm) between the groups, with an increased quantity of sEVs in patients with APS and aPLneg IT compared to HBD. MACSPlex analysis of 37 different sEV surface markers showed endothelial (CD31), platelet (CD41b and CD42a), leukocyte (CD45), CD8 lymphocyte and APC (HLA-ABC) cell-derived sEVs. Except for CD8, these molecules were comparably expressed in all study groups. sEVs from APS patients were specifically enriched in surface expression of CD62P, suggesting endothelial and platelet activation in APS. Additionally, APS patients exhibited increased CD133/1 expression compared to aPL-neg IT, suggesting endothelial damage in APS patients. These findings demonstrate enhanced shedding, and distinct biological properties of sEVs in thrombotic APS. antiphospholipid syndrome thrombosis adhesion molecules small extracellular vesicles surface protein markers endothelial activation platelet activation antifosfolipidni sindrom tromboza adhezijske molekule true false true Angleški jezik Slovenski jezik Članek v reviji 2021-12-13 11:18:31 2021-12-13 11:18:34 2022-09-13 03:52:25 0000-00-00 00:00:00 2020 0 0 13 str. iss. 5, art. 1211 Vol. 9 May 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1 2073-4409 10.3390/cells9051211 15921155 RAZ_Stok_Ula_2020.pdf RAZ_Stok_Ula_2020.pdf 1 F0BE55C65422BAC48D5B0FA599429C6F a861fb5d17a02aa46671ac27e7c4f4a7c3e8ab19d4aa88f4b3ab4668512a0e89 68c645e9-5bfe-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152049 https://www.mdpi.com/2073-4409/9/5/1211 1 e96b2de7-5bfd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152048 Fakulteta za farmacijo Medicinska fakulteta 0 0 0