20.500.12556/RUL-133718 Escherichia coli isolated from cases of colibacillosis in Russian poultry farms (Perm Krai) sensitivity to antibiotics and bacteriocins Escherichia coli strains isolated from case of colibacillosis in Russian poultry farms in the region of Perm Krai were analyzed for their sensitivity to main antibiotics and bacteriocins. Sensitivity profiles for 9 antibiotics and 20 bacteriocins were determined with the disc diffusion method and the overlay test, respectively. Further, with the PCR the presence of several bla and integron 1 genes was revealed and the phylogenetic group for each strain determined. Among the 28 studied E. coli strains 85.7% were resistant to at least three antibiotics, 53.6% to five or more drugs, and 10.7% to eight antibiotics. PCR revealed that the bla$_{TEM}$ gene was harbored by 71.4% of strains and the bla$_{CTX-M}$ gene by 53.6% of strains. The class 1 integrons were found in 28.6% of strains. All of the studied strains were insensitive to ten or more bacteriocins. More than 90% of the studied strains were insensitive to pore-forming colicins of group A and B colicins, while 60.7% were insensitive to colicins with DNase and RNase activity. All of the analyzed strains were insensitive to at least two of the tested microcins. Neither the antibiotic resistance profile nor the bacteriocin resistance profile correlated with phylogenetic group of the strains. Thus, the studied strains were shown to possess high levels of multiple resistance to antibiotics and insensitivity to bacteriocins. avian Escherichia coli antibiotics bacteriocins colicins microcins ptičja Escherichia coli antibiotiki bakteriocini kolicini mikrocini true false true Angleški jezik Slovenski jezik Članek v reviji 2021-12-10 13:56:50 2021-12-10 13:56:51 2022-09-13 03:52:18 0000-00-00 00:00:00 2020 0 0 11 str. iss. 5, art. 741 Vol. 8 May 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2076-2607 10.3390/microorganisms8050741 16277507 RAZ_Kuznetsova_Marina_V._2020.pdf RAZ_Kuznetsova_Marina_V._2020.pdf 1 E97D9E7FA2942A909E7A09D7176E25CB 84471c52be6f141cc6adc1e79db99143fbfa96386f2d5126557c1b14bb0f5db5 c0ab34fd-59b8-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152019 https://www.mdpi.com/2076-2607/8/5/741 1 879b9291-59b8-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=152018 Biotehniška fakulteta 0 0 0