20.500.12556/RUL-133415 Comparison of selection methods for the establishment of a core collection using SSR markers for hazelnut (Corylus avellana L.) accessions from European germplasm repositories Hazelnut (Corylus avellana L.) is one of the most important tree nut crops in Europe. Germplasm accessions are conserved in ex situ repositories, located in countries where hazelnut production occurs. In this work, we used ten simple sequence repeat (SSR) markers as the basis to establish a core collection representative of the hazelnut genetic diversity conserved in different European collections. A total of 480 accessions were used: 430 from ex situ collections and 50 landraces maintained on-farm. SSR analysis identified 181 genotypes, that represented our whole hazelnut germplasm collection (WHGC). Four approaches (utilizing MSTRAT, Power Core, and Core Hunter%s single- and multi-strategy) based on the maximization (M) strategy were used to determine the best sampling method. Core Hunter%s multi-strategy, optimizing both allele coverage (Cv) and Cavalli-Sforza and Edwards (Dce) distance with equal weight, outperformed the others and was selected as the best approach. The final core collection (Cv-Dce30) comprised 30 entries (16.6% of genotypes). It recovered all SSR alleles and preserved parameter variations when compared to WHGC. Entries represented all six gene pools obtained from the population structure analysis of WHGC, further confirming the representativeness of Cv-Dce30. Our findings contribute towards improving the conservation and management of European hazelnut genetic resources and could be used to optimize future research by identifying a minimum number of accessions on which to focus. hazelnut corylus avellana plant genetic resources germplasm management SSR markers microsatellites germplasm repositories in situ conservation ex situ conservation leska corylus avellana genska banka mikrosatelitski markerji genotipi true false true Angleški jezik Slovenski jezik Neznano 2021-11-26 09:19:13 2021-11-26 09:19:18 2022-09-12 04:16:15 0000-00-00 00:00:00 2021 0 0 Str. 1-14 (48) Iss. 6 Vol. 17 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-11-19 634.5 1614-2942 10.1007/s11295-021-01526-7 86288899 RAZ_Boccacci_Paolo_2021.pdf RAZ_Boccacci_Paolo_2021.pdf 1 9CCC41D8C63F262ACFDFFCDD861EE133 f62bec61758ccba2975be43094e3a55ae12543acfbe8cd70d3f28a8dd4d5a160 d68f264b-4e92-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151460 Biotehniška fakulteta 0 0 0