20.500.12556/RUL-133403 The neurotropic black yeast Exophiala dermatitidis induces neurocytotoxicity in neuroblastoma cells and progressive cell death The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits diverse indoor environments, in particular bathrooms, steam baths, and dishwashers. Here, we show that the selected strain, EXF-10123, is polymorphic, can grow at 37 °C, is able to assimilate aromatic hydrocarbons (toluene, mineral oil, n-hexadecane), and shows abundant growth with selected neurotransmitters (acetylcholine, gamma-aminobutyric acid, glycine, glutamate, and dopamine) as sole carbon sources. We have for the first time demonstrated the effect of E. dermatitidis on neuroblastoma cell model SH-SY5Y. Aqueous and organic extracts of E. dermatitidis biomass reduced SH-SY5Y viability by 51% and 37%, respectively. Melanized extracellular vesicles (EVs) prepared from this strain reduced viability of the SH-SY5Y to 21%, while non-melanized EVs were considerably less neurotoxic (79% viability). We also demonstrated direct interactions of E. dermatitidis with SH-SY5Y by scanning electron and confocal fluorescence microscopy. The observed invasion and penetration of neuroblastoma cells by E. dermatitidis hyphae presumably causes the degradation of most neuroblastoma cells in only three days. This may represent a so far unknown indirect or direct cause for the development of some neurodegenerative diseases such as Alzheimer's. neurotropic fungi black yeasts neurotropism hydrocarbons extracellular vesicles Alzheimer’s disease human neuroblastoma cells SH-SY5Y Exophiala dermatitidis nevrotropne glive črne kvasovke nevrotropizem true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-25 11:56:38 2021-11-25 11:56:40 2022-09-12 04:16:11 0000-00-00 00:00:00 2020 0 0 22 str. iss. 4, art. 963 Vol. 9 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2073-4409 10.3390/cells9040963 34781401 RAZ_Lavrin_Teja_2020.pdf RAZ_Lavrin_Teja_2020.pdf 1 EEEFC3A0CE2470F2A245C1A5A8622F6A 63c428565440b1c4939fc1e09fa24959aac401036029e2491a6d22996033bbbd 82cf456e-4dde-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151440 https://www.mdpi.com/2073-4409/9/4/963 1 48c15e69-4dde-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151439 Biotehniška fakulteta Medicinska fakulteta Veterinarska fakulteta Fakulteta za kemijo in kemijsko tehnologijo 0 0 0