20.500.12556/RUL-133368 Electronic emulator of biological tissue as an electrical load during electroporation Electroporation is an emerging technology, with great potential in many different medical and biotechnological applications, food engineering and biomass processing. Large variations of biological load characteristics, however, represent a great challenge in electroporator design, which results in different solutions. Because a clinical electroporator is a medical device, it must comply with medical device regulative and standards. However, none of the existing standards directly address the operation or electroporator’s performance requirements. In order to evaluate clinical, laboratory and prototype electroporation devices during the development process, or to evaluate their final performance considering at least from the perspective of output pulse parameters, we present a case study on the design of an electronic emulator of biological tissue as an electrical load during electroporation. The proposed electronic load emulator is a proof of concept, which enables constant and sustainable testing and unbiased comparison of different electroporators’ operations. We developed an analog electrical circuit that has equivalent impedance to the beef liver tissue in combination with needle electrodes, during high voltage pulse delivery and/or electroporation. Current and voltage measurements during electroporation of beef liver tissue ex vivo, were analyzed and parametrized to define the analog circuit equation. An equivalent circuit was simulated, built and validated. The proposed concept of an electronic load emulator can be used for “classical” electroporator (i.e., not nanosecond) performance evaluation and comparison of their operation. Additionally, it facilitates standard implementation regarding the testing protocol and enables quality assurance. electroporation tissue conductivity change standard nonlinear load biological load electroporation emulator elektroporacija sprememba prevodnosti tkiva standardno nelinearno breme biološko breme emulator elektroporacije true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-24 10:21:02 2021-11-24 10:21:03 2022-09-12 04:16:01 0000-00-00 00:00:00 2020 0 0 15 str. iss. 9, art. 3103 Vol. 10 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.621 2076-3417 10.3390/app10093103 13261059 RAZ_Pirc_Eva_2020.pdf RAZ_Pirc_Eva_2020.pdf 1 9A78FD59A453269FD53C429310C80309 91dc6c2240377780e4036165cea8e23c265c0c4fc3444bb423ac9c24fc07105d 01b2815e-4d08-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151383 https://www.mdpi.com/2076-3417/10/9/3103 1 c38bae74-4d07-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151382 Fakulteta za elektrotehniko 0 0 0