20.500.12556/RUL-133267 Mineral element composition in grain of awned and awnletted wheat (Triticum aestivum L.) cultivars tissue-specific iron speciation and phytate and non-phytate ligand ratio In wheat (Triticum aestivum L.), the awns—the bristle-like structures extending from lemmas—are photosynthetically active. Compared to awned cultivars, awnletted cultivars produce more grains per unit area and per spike, resulting in significant reduction in grain size, but their mineral element composition remains unstudied. Nine awned and 11 awnletted cultivars were grown simultaneously in the field. With no difference in 1000-grain weight, a larger calcium and manganese—but smaller iron (Fe) concentrations—were found in whole grain of awned than in awnletted cultivars. Micro X-ray absorption near edge structure analysis of different tissues of frozen-hydrated grain cross-sections revealed that differences in total Fe concentration were not accompanied by differences in Fe speciation (64% of Fe existed as ferric and 36% as ferrous species) or Fe ligands (53% were phytate and 47% were non-phytate ligands). In contrast, there was a distinct tissue-specificity with pericarp containing the largest proportion (86%) of ferric species and nucellar projection (49%) the smallest. Phytate ligand was predominant in aleurone, scutellum and embryo (72%, 70%, and 56%, respectively), while nucellar projection and pericarp contained only non-phytate ligands. Assuming Fe bioavailability depends on Fe ligands, we conclude that Fe bioavailability from wheat grain is tissue specific. biofortification phytate iron awn X-ray fluorescence X-ray absorption spectrometry phosphorus sulphur nicotianamine true false true Angleški jezik Ni določen Članek v reviji 2021-11-19 07:58:35 2021-11-19 07:58:36 2022-09-12 04:15:33 0000-00-00 00:00:00 2020 0 0 14 str. iss. 1, art. 79 Vol. 9 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 53 2223-7747 10.3390/plants9010079 33018919 RAZ_Pongrac_Paula_2020.pdf RAZ_Pongrac_Paula_2020.pdf 1 3F980BB92E35D6CDB7E9E8136D54C99B 63703e20e7d442a984de30b36bcbbd20e358f25867d32f7a8814c42eed407ed2 64c8c17f-4906-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151175 https://www.mdpi.com/2223-7747/9/1/79 0 494e2664-4906-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=151174 Biotehniška fakulteta 0 0 0