20.500.12556/RUL-133136 Geographical variability affects CCHFV detection by RT-PCR a tool for in-silico evaluation of molecular assays The Crimean-Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according to the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. The high genetic variability of CCHFV often hampers the efficacy of available molecular tests and can affect their diagnostic potential. Recently, increasing numbers of complete CCHFV genomic sequences have become available, allowing a better appreciation of the genomic evolution of this virus. We summarized the current knowledge on molecular methods and developed a new bioinformatics tool to evaluate the existing assays for CCHFV detection, with a special focus on strains circulating in different geographical areas. Twenty-two molecular methods and 181 sequences of CCHFV were collected, respectively, from PubMed and GenBank databases. Up to 28 mismatches between primers and probes of each assay and CCHFV strains were detected through in-silico PCR analysis. Combinations of up to three molecular methods markedly decreased the number of mismatches within most geographic areas. These results supported the good practice of CCHFV detection of performing more than one assay, aimed for different sequence targets. The choice of the most appropriate tests must take into account patient's travel history and geographic distribution of the different CCHFV strains. Crimean-Congo hemorrhagic fever molecular detection CCHFV Crimean–Congo hemorrhagic fever virus arthropod-borne virus laboratory preparedness emerging diseases krimsko-kongoška hemoragična mrzlica molekularno odkrivanje arbovirus true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-12 11:22:42 2021-11-12 11:22:45 2022-09-12 04:15:02 0000-00-00 00:00:00 2019 0 0 14 str. iss. 10, art. 953 Vol. 11 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 1999-4915 10.3390/v11100953 34533081 RAZ_Gruber_Cesare_EM_2019.pdf RAZ_Gruber_Cesare_EM_2019.pdf 1 729D1DAE0D60D051544084789E1BE778 331fc2cd3dca59d9c216d035e81dd2c760b2d3a7a6e2ec9d6042e7c04352be76 9828b55f-43a2-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150958 https://www.mdpi.com/1999-4915/11/10/953 1 6a795dcd-43a2-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150957 Medicinska fakulteta 0 0 0