20.500.12556/RUL-133113 CNL–Clitocybe nebularis lectin—the fungal GalNAcβ1-4GlcNAc-binding lectin Clitocybe nebularis lectin (CNL) is present in fruiting bodies of clouded agaric along with several similar isolectins that are all small and stable proteins. It is a beta-trefoil type lectin forming homodimers that are essential for its functionality. It binds specifically N,N′-diacetyllactosediamine (GalNAcβ1-4GlcNAc, LacDiNac) and human blood group A determinant-containing glycan epitopes. Its most probable function is to defend fruiting bodies against predators and parasites. In addition, an endogenous regulatory function is possible for CNL, as indicated by its interaction with fungal protease inhibitors sharing the beta-trefoil fold. CNL is toxic to insects, nematodes and amoebae, as well as to leukemic T-cell lines. Bivalent carbohydrate binding is essential for the toxicity of CNL, against both invertebrates and cancer-derived cell lines. In addition, CNL exhibits potent immunostimulation of human dendritic cells, resulting in a strong T helper cell type 1 response. Based on its unique characteristics, CNL is a promising candidate for applications in human and veterinary medicine as well as in agriculture, for plant protection. beta-trefoil fold LacDiNAc entomotoxic leukemic T-cell antiproliferative dendritic cell true false true Angleški jezik Ni določen Članek v reviji 2021-11-11 12:26:02 2021-11-11 12:26:02 2022-09-12 04:14:55 0000-00-00 00:00:00 2019 0 0 10 str. iss. 23, art. 4204 Vol. 24 2109 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1420-3049 10.3390/molecules24234204 32898599 RAZ_Sabotic_Jerica_2019.pdf RAZ_Sabotic_Jerica_2019.pdf 1 2CE0B101F35CC9761982D894CB105DF7 dedebac9a3b858d5585e26929dc9fb9c81bbba87a2f645de7aea7d89027eea2e 4e264b02-42e2-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150919 https://www.mdpi.com/1420-3049/24/23/4204 0 3b57664a-42e2-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150918 Fakulteta za farmacijo 0 0 0