20.500.12556/RUL-133063 The anti-Campylobacter activity and mechanisms of pinocembrin action We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic and phenotypic levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168∆cmeR). At its minimal inhibitory concentration, pinocembrin significantly increased cell membrane permeability of Campylobacter. Interestingly, at sub-inhibitory concentrations, pinocembrin did not significantly alter membrane functionality and it increased bacterial fitness. Treatment with pinocembrin evoked decreased expression of ribosomal proteins and down-regulation of several NADH dehydrogenase I chain subunits and proteins involved in iron uptake. This suggests altered protein production and redox cycle and iron metabolism. Interestingly, the chelation of Fe ions during the treatment with pinocembrin increased C. jejuni survival, although there was no increase in the formation of reactive oxygen species. Pre-treatment of C. jejuni with sub-inhibitory concentrations of pinocembrin for 2 h resulted in a 1 log decrease in C. jejuni colony forming units in mice liver at 8 days post-infection, compared to untreated C. jejuni. These findings suggest that pinocembrin modulates the metabolic activity of C. jejuni and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. This anti-Campylobacter potential of pinocembrin warrants further investigation. Campylobacter jejuni pinocembrin mechanism of antibacterial activity gene expression virulence Campylobacter jejuni pinocembrin protimikrobne snovi protimikrobna aktivnost patogeni mikroorganizmi true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-10 09:03:16 2021-11-10 09:03:16 2022-09-12 04:14:40 0000-00-00 00:00:00 2019 0 0 15 str. iss. 12, art. 675 Vol. 7 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24:615.33 2076-2607 10.3390/microorganisms7120675 5133688 RAZ_Klancnik_Anja_2019.pdf RAZ_Klancnik_Anja_2019.pdf 1 48527B531C34D2843C1F69A448AFC260 03e67aff255a5417bca1ac4efa148631e6384657c0eefba1234c0a0a591bd6cd 3c9ddb6d-41fd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150834 https://www.mdpi.com/2076-2607/7/12/675 0 e2fd6c29-41fc-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150833 Biotehniška fakulteta 0 0 0