20.500.12556/RUL-132852 Characterization of biomarker levels in Crimean-Congo hemorrhagic fever and hantavirus fever with renal syndrome Hemorrhagic fever with renal syndrome (HFRS) and Crimean-Congo hemorrhagic fever (CCHF) are important viral hemorrhagic fevers (VHF), especially in the Balkan region. Infections with Dobrava or Puumala orthohantavirus and Crimean-Congo hemorrhagic fever orthonairovirus can vary from a mild, nonspecific febrile illness, to a severe disease with a fatal outcome. The pathogenesis of both diseases is poorly understood, but it has been suggested that a host's immune mechanism might influence the pathogenesis of the diseases and survival. The aim of our study is to characterize cytokine response in patients with VHF in association with the disease progression and viral load. Forty soluble mediators of the immune response, coagulation, and endothelial dysfunction were measured in acute serum samples in 100 HFRS patients and 70 CCHF patients. HFRS and CCHF patients had significantly increased levels of IL-6, IL-12p70, IP-10, INF-γ, TNF-α, GM-CSF, MCP-3, and MIP-1b in comparison to the control group. Interestingly, HFRS patients had higher concentrations of serum MIP-1α, MIP-1β, which promote activation of macrophages and NK cells. HFRS patients had increased concentrations of IFN-γ and TNF-α, while CCHF patients had significantly higher concentrations of IFN-α and IL-8. In both, CCHF and HFRS patients' viral load significantly correlated with IP-10. Patients with fatal outcome had significantly elevated concentrations of IL-6, IFN-α2 and MIP-1α, while GRO-α, chemokine related to activation of neutrophils and basophils, was downregulated. Our study provided a comprehensive characterization of biomarkers released in the acute stages of CCHF and HFRS. Crimean-Congo hemorrhagic fever hantavirus fever biomarkers VHF CCHF HFRS cytokines krimsko-kongoška hemoragična mrzlica hantavirusna mrzlica biomarkerji true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-05 09:23:24 2021-11-05 09:23:28 2022-09-12 04:14:02 0000-00-00 00:00:00 2019 0 0 19 str. iss. 8, art. 686 Vol. 11 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 1999-4915 10.3390/v11080686 34443481 RAZ_Korva_Misa_2019.pdf RAZ_Korva_Misa_2019.pdf 1 A17BF948BCC8D097AEFFD924A0274092 f3acaed4697d8747b4d1695696595d639925a7a772064420a31f408088df5f29 e8c00efe-3e12-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150584 https://www.mdpi.com/1999-4915/11/8/686 0 9b4a28b6-3e11-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150583 Medicinska fakulteta 0 0 0