20.500.12556/RUL-132807 Viscosity of plasma as a key factor in assessment of extracellular vesicles by light scattering Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity. extracellular vesicles exosomes blood plasma dynamic light scattering static light scattering shape characterization viscosity of blood plasma izvencelični vezikli eksosomi krvna plazma dinamično sipanje svetlobe statično sipanje svetlobe določanje oblike viskoznost krvne plazme true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-03 16:15:42 2021-11-03 16:15:43 2022-09-12 04:13:50 0000-00-00 00:00:00 2019 0 0 21 str. iss. 9, art. 1046 Vol. 8 Sep. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.35 2073-4409 10.3390/cells8091046 1538312131 RAZ_Bozic_Darja_2019.pdf RAZ_Bozic_Darja_2019.pdf 1 05179C0E1F0114206C00B5056480E4ED c59fdfd5e57d713a0e7708bef520b4813e01d9ead6d684403f36ee08e0eefc56 0509b27f-3cb9-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150519 https://www.mdpi.com/2073-4409/8/9/1046 1 de4489e3-3cb8-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150518 Fakulteta za kemijo in kemijsko tehnologijo Zdravstvena fakulteta 0 0 0