20.500.12556/RUL-132789 Antibiotic resistance, virulence factors, phenotyping, and genotyping of E. coli isolated from the feces of healthy subjects Escherichia coli may innocuously colonize the intestine of healthy subjects or may instigate infections in the gut or in other districts. This study investigated intestinal E. coli isolated from 20 healthy adults. Fifty-one strains were genotyped by molecular fingerprinting and analyzed for genetic and phenotypic traits, encompassing the profile of antibiotic resistance, biofilm production, the presence of surface structures (such as curli and cellulose), and their performance as recipients in conjugation experiments. A phylogroup classification and analysis of 34 virulence determinants, together with genes associated to the pks island (polyketide-peptide genotoxin colibactin) and conjugative elements, was performed. Most of the strains belonged to the phylogroups B1 and B2. The different phylogroups were separated in a principal coordinate space, considering both genetic and functional features, but not considering pulsed-field gel electrophoresis. Within the B2 and F strains, 12 shared the pattern of virulence genes with potential uropathogens. Forty-nine strains were sensitive to all the tested antibiotics. Strains similar to the potential pathogens innocuously inhabited the gut of healthy subjects. However, they may potentially act as etiologic agents of extra-intestinal infections and are susceptible to a wide range of antibiotics. Nevertheless, there is still the possibility to control infections with antibiotic therapy. Escherichia coli typing gut microbiota PFGE pulsed-field gel electrophoresis virulence antibiotic resistance conjugation curli co-occurrence true false true Angleški jezik Ni določen Članek v reviji 2021-11-03 11:00:31 2021-11-03 11:00:31 2022-09-12 04:13:44 0000-00-00 00:00:00 2019 0 0 18 str. iss. 8, art. 251 Vol. 7 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-2607 10.3390/microorganisms7080251 5151311 RAZ_Raimondi_Stefano_2019.pdf RAZ_Raimondi_Stefano_2019.pdf 1 E47AF68699A31F190EC5A3B06ED313A1 3430272d1c5c996b47d1e23ec68d8a3ac582ffd3273762eb46251e93ee228799 23f1a90b-3c8d-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150495 https://www.mdpi.com/2076-2607/7/8/251 0 17ad4e85-3c8d-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150494 Biotehniška fakulteta 0 0 0