20.500.12556/RUL-132781 Anthocyanins protect hepatocytes against CCl$_4$-induced acute liver injury in rats by inhibiting pro-inflammatory mediators, polyamine catabolism, lipocalin-2, and excessive proliferation of Kupffer cells This study examined the hepatoprotective and anti-inflammatory effects of anthocyanins from Vaccinim myrtillus (bilberry) fruit extract on the acute liver failure caused by carbon tetrachloride-CCl$_4$ (3 mL/kg, i.p.). The preventive treatment of the bilberry extract (200 mg anthocyanins/kg, orally, 7 days) prior to the exposure to the CCl$_4$ resulted in an evident decrease in markers of liver damage (glutamate dehydrogenase, sorbitol dehydrogenase, malate dehydrogenase), and reduced pro-oxidative (conjugated dienes, lipid hydroperoxide, thiobarbituric acid reactive substances, advanced oxidation protein products, NADPH oxidase, hydrogen peroxide, oxidized glutathione), and pro-inflammatory markers (tumor necrosis factor-alpha, interleukin-6, nitrite, myeloperoxidase, inducible nitric oxide synthase, cyclooxygenase-2, CD68, lipocalin-2), and also caused a significant decrease in the dissipation of the liver antioxidative defence capacities (reduced glutathione, glutathione S-transferase, and quinone reductase) in comparison to the results detected in the animals treated with CCl$_4$ exclusively. The administration of the anthocyanins prevented the arginine metabolism’s diversion towards the citrulline, decreased the catabolism of polyamines (the activity of putrescine oxidase and spermine oxidase), and significantly reduced the excessive activation and hyperplasia of the Kupffer cells. There was also an absence of necrosis, in regard to the toxic effect of CCl$_4$ alone. The hepatoprotective mechanisms of bilberry extract are based on the inhibition of pro-oxidative mediators, strong anti-inflammatory properties, inducing of hepatic phase II antioxidant enzymes (glutathione S-transferase, quinone reductase) and reduced glutathione, hypoplasia of Kupffer cells, and a decrease in the catabolism of polyamines. carbon tetrachloride anthocyanins anti-inflammatory effects Kupffer cells lipocalin-2 polyamine catabolism rastlinski izvlečki antioksidanti antociani bolezni jeter protivnetno delovanje true false true Angleški jezik Slovenski jezik Članek v reviji 2021-11-03 09:48:35 2021-11-03 09:48:37 2022-09-12 04:13:42 0000-00-00 00:00:00 2019 0 0 23 str. iss. 10, art. 451 Vol. 8 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.36:577.1:547.973 2076-3921 10.3390/antiox8100451 5107576 RAZ_Popovic_Dejan_2019.pdf RAZ_Popovic_Dejan_2019.pdf 1 64485D17A5108415874B175ADEAD6112 61d8ad1b56f8c593511e08d076404d14867e8e08ea01d88d284ca84a42f67e82 bb81dfe2-3c83-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150481 https://www.mdpi.com/2076-3921/8/10/451 0 b60d44cf-3c83-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150480 Biotehniška fakulteta 0 0 0