20.500.12556/RUL-132520 Vpliv hladne atmosferske plazme na virus blage lisavosti paprike magistrsko delo The effect of cold atmospheric plasma on pepper mild mottle virus. magistrski študij - 2. stopnja V naši raziskovalni nalogi smo preučevali vpliv hladne atmosferske plazme na virus blage lisavosti paprike (PMMoV; angl. pepper mild mottle virus). Želeli smo ugotoviti, če je hladna atmosferska plazma primerna metoda za inaktivacijo PMMoV v vodi. PMMoV je rastlinski patogen, ki se v odpadnih vodah nahaja v visokih koncentracijah in ga je s tradicionalnimi metodami za čiščenje vode težko inaktivirati. Poleg tega imajo lahko tradicionalne metode določene negativne lastnosti kot so visoka cena in nastanek škodljivih produktov. Po obdelavi vzorcev vode, ki je vsebovala PMMoV, s hladno atmosfersko plazmo, smo na testnih rastlinah izvedli teste infektivnosti, prisotnost PMMoV v testnih rastlinah pa smo preverili z verižno reakcijo s polimerazo z reverzno transkripcijo v realnem času. Vpliv hladne atmosferske plazme na virusno RNK oz. njeno razgradnjo smo preverili z verižno reakcijo s polimerazo z reverzno transkripcijo, namnožene produkte pa smo določali z gelsko elektroforezo. Pokazali smo, da je PMMoV mogoče inaktivirati že po pet minutni obdelavi s hladno atmosfersko plazmo, v tem času pa je prišlo tudi do razgradnje RNK. Zaradi omenjenega, bi hladna atmosferska plazma v prihodnosti lahko predstavljala pomembno orodje za čiščenje odpadnih voda in inaktivacijo prisotnih patogenov. In our research, we investigated the effect of cold atmospheric plasma on pepper mild mottle virus (PMMoV). The aim was to find out if cold atmospheric plasma is a suitable method to inactivate PMMoV in water. PMMoV is a plant pathogen that is found in high concentrations in wastewater and is difficult to inactivate using conventional water treatment methods. Moreover, these methods may have some limitations, such as high cost and the formation of harmful by-products. After treating water samples containing PMMoV with cold atmospheric plasma, infectivity assays were performed on test plants. The presence of PMMoV in the test plants was determined by reverse transcription real-time polymerase chain reaction. The effect of cold atmospheric plasma on viral RNA degradation was determined by reverse-transcription polymerase chain reaction, and the amplified products were detected by gel electrophoresis. We have shown that it is possible to inactivate PMMoV after only 5 min of treatment with cold atmospheric plasma, during which time RNA was also degraded. Therefore, cold atmospheric plasma could be an important tool for wastewater treatment and inactivation of existing pathogens in the future. Rastlinski virusi tobamovirusi virus blage lisavosti paprike hladna atmosferska plazma dekontaminacija vode verižna reakcija s polimerazo Plant viruses tobamoviruses pepper mild mottle virus cold atmospheric pressure plasma water decontamination polymerase chain reaction true false false [U. Škripec] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2021-10-28 07:15:02 2021-10-28 07:15:07 2022-09-12 04:12:40 0000-00-00 00:00:00 2021 0 Ljubljana 0 Ljubljana X f., 39 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2(043.2) 199715 83347203 21557.pdf 21557.pdf 1 23B92B606BCBE6EFD14799A7B688DFCD b4888a7d9064e55813a402ba7a93f73380d5dd3413b76240c1deb6096adef87c fe4a8339-37ad-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150178 Biotehniška fakulteta 0 0 0