20.500.12556/RUL-132350 Insect protein-based diet as potential risk of allergy in dogs Before insects can be used widely as an alternative source of dietary protein, their allerginicity should be investigated. Therefore, the aim of our study was to assess the potential adverse reactions of the immune system of dogs against Tenebrio molitor proteins. Dogs sensitised to storage mites T. putrescentiae and A. siro were included. Clinically healthy and clinically allergic dogs were compared. Proteins were extracted from mealworm larvae and their digestibility determined by in vitro incubation with digestive proteases. Mealworm protein extracts and digests were analysed by SDS–PAGE. Canine sera tested for the presence of mite-specific IgEs were used for subsequent Western blotting. LC-MS/MS analysis was used to identify mealworm proteins and their allergenic potential was predicted with the AllermatchTM tool. The binding of canine sera IgEs to mealworm proteins was confirmed; however, the differences between the two groups of dogs were not significant. Moreover, no clear correlation was found between sensitisation to storage mites and clinical status of the dogs. Altogether, 17 different proteins were identified, including tropomyosin, α-amylase, and Tm-E1a cuticular protein that are known cross-reacting IgE-binding allergens. Our results suggest that dogs allergic to mites may clinically express also the cross-reactivity with mealworm proteins. dogs allergy mites mealworm proteomics hypersensitivity Tenebrio true false true Angleški jezik Ni določen Članek v reviji 2021-10-22 09:14:14 2021-10-22 09:14:15 2022-09-12 04:12:03 0000-00-00 00:00:00 2021 0 0 16 str. iss. 7, art. 1942 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57 2076-2615 10.3390/ani11071942 68769795 RAZ_Premrov_Bajuk_Blanka_2021.pdf RAZ_Premrov_Bajuk_Blanka_2021.pdf 1 2AEF41F92F42A55EC483A93AB4FACACE 23bc509d2321951caadd115e9ef9ebe9c0321ec43001dc5a562e1a5cec9e9d59 316f98f4-a6a5-11ec-960c-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154681 https://www.mdpi.com/2076-2615/11/7/1942 1 a5fbb037-3307-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149966 Veterinarska fakulteta 0 0 0