20.500.12556/RUL-132325 Revisiting the role of transcription factors in coordinating the defense response against Citrus bark cracking viroid infection in commercial hop (Humulus lupulus L.) Transcription factors (TFs) play a major role in controlling gene expression by intricately regulating diverse biological processes such as growth and development, the response to external stimuli and the activation of defense responses. The systematic identification and classification of TF genes are essential to gain insight into their evolutionary history, biological roles, and regulatory networks. In this study, we performed a global mining and characterization of hop TFs and their involvement in Citrus bark cracking viroid CBCVd infection by employing a digital gene expression analysis. Our systematic analysis resulted in the identification of a total of 3,818 putative hop TFs that were classified into 99 families based on their conserved domains. A phylogenetic analysis classified the hop TFs into several subgroups based on a phylogenetic comparison with reference TF proteins from Arabidopsis thaliana providing glimpses of their evolutionary history. Members of the same subfamily and subgroup shared conserved motif compositions. The putative functions of the CBCVd-responsive hop TFs were predicted using their orthologous counterparts in A. thaliana. The analysis of the expression profiling of the CBCVd-responsive hop TFs revealed a massive differential modulation, and the expression of the selected TFs was validated using qRT-PCR. Together, the comprehensive integrated analysis in this study provides better insights into the TF regulatory networks associated with CBCVd infections in the hop, and also offers candidate TF genes for improving the resistance in hop against viroids. transcriptional reprogramming viroid pathogenesis hop stunt disease management genetske transformacije viroid razpokanosti skorje agrumov CBCVd huda viroidna zakrnelost hmelja Humulus lupulus hmelj transkriptomska analiza različno izraženi geni true false true Angleški jezik Slovenski jezik Članek v reviji 2021-10-21 13:25:43 2021-10-21 13:25:44 2022-09-12 04:11:55 0000-00-00 00:00:00 2019 0 0 19 str. iss. 5, art. 419 Vol. 11 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1999-4915 10.3390/v11050419 9229433 RAZ_Nath_Vishnu_Sukumari_2019.pdf RAZ_Nath_Vishnu_Sukumari_2019.pdf 1 999E2647548A749809BF8CEA84AA2345 f7b129d804410d46eedc3c75f042c9defeaf1b136b2f383b0642fb050eb3076f d7d34320-3261-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149927 https://www.mdpi.com/1999-4915/11/5/419 0 d13812a2-3261-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149926 Biotehniška fakulteta 0 0 0