20.500.12556/RUL-132160 Contrast-enhanced ultrasound for evaluation of tumor perfusion and outcome following treatment in a murine melanoma model Due to a lack of data on predictors of electroporation-based treatment outcomes, we investigated the potential predictive role of contrast-enhanced harmonic ultrasound (CEUS) in mice B16F10 melanoma treated by gene electrotransfer (GET) to silence melanoma cell adhesion molecule (MCAM) and radiotherapy, which has not been evaluated yet. CEUS evaluation was verified by tumor histological analysis. Mice bearing subcutaneous tumors were treated with GET to silence MCAM, irradiation or the combination of GET to silence MCAM and irradiation (combined treatment). CEUS of the tumors used to evaluate tumor perfusion was performed before and up to 10 days after the beginning of the experiment, and the CEUS results were compared with tumor growth and the number of blood vessels analyzed in the histological tumor sections. CEUS revealed a decrease in tumor perfusion in the combined therapy groups compared with the control groups and correlated with tumor histological analyses, which showed a decreased vascular density. In this study a trend of inverse correlation was observed between tumor perfusion and treatment efficacy. The greater the perfusion of the tumor, the shorter the expected doubling time. Furthermore, decreased perfusion showed a trend to correlate with higher antitumor efficacy. Thus, CEUS could be used to predict tumoral vascular density and treatment effectiveness. murine melanoma B16F10 gene electrotransfer melanoma cell adhesion molecule irradiation antivascular effects contrast-enhanced harmonic ultrasound melanoma electrochemotherapy ultrasonography mice true false true Angleški jezik Ni določen Članek v reviji 2021-10-14 09:24:28 2021-10-14 09:24:29 2022-09-12 04:11:18 0000-00-00 00:00:00 2021 0 0 9 str. art. 107932 Vol. 142 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 1878-562X 10.1016/j.bioelechem.2021.107932 74631939 RAZ_Brloznik_Maja_2021.pdf RAZ_Brloznik_Maja_2021.pdf 1 1F6E896D325B1EE6FBBB4512D26F87D9 356ec4125d6ff2c4ef95564c4ed56c2c7540d63e5664acb1d53289044115ecf9 8590dde9-2cc0-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149702 https://www.sciencedirect.com/science/article/pii/S156753942100195X 0 c42ecd32-2cbf-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149701 Veterinarska fakulteta Medicinska fakulteta Zdravstvena fakulteta 0 0 0