20.500.12556/RUL-131726 The biological fate of silver nanoparticles from a methodological perspective We analyzed the performance and throughput of currently available analytical techniques for quantifying body burden and cell internalization/distribution of silver nanoparticles (Ag NPs). Our review of Ag NP biological fate data shows that most of the evidence gathered for Ag NPs body burden actually points to total Ag and not only Ag NPs. On the other hand, Ag NPs were found inside the cells and tissues of some organisms, but comprehensive explanation of the mechanism(s) of NP entry and/or in situ formation is usually lacking. In many cases, the methods used to detect NPs inside the cells could not discriminate between ions and particles. There is currently no single technique that would discriminate between the metals species, and at the same time enable localization and quantification of NPs down to the cellular level. This paper serves as an orientation towards selection of the appropriate method for studying the fate of Ag NPs in line with their properties and the specific question to be addressed in the study. Guidance is given for method selection for quantification of NP uptake, biodistribution, precise tissue and cell localization, bioaccumulation, food chain transfer and modeling studies regarding the optimum combination of methods and key factors to consider. silver nanoparticles review body burden biodistribution internalization bioaccumulation microscopy spectroscopy throughput performance quantification true false true Angleški jezik Ni določen Članek v reviji 2021-10-01 14:41:19 2021-10-01 14:41:20 2022-09-10 04:13:24 0000-00-00 00:00:00 2018 0 0 12 str. iss. 6, art. 957 Vol. 11 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 620.3 1996-1944 10.3390/ma11060957 4711247 RAZ_Drobne_Damjana_2018.pdf RAZ_Drobne_Damjana_2018.pdf 1 89054580F0C697D0749F080FC83330E6 0731adbd88f7788cf35e881a89b8093819d1ac0b1b638602c6069cdfb1be337b f766a001-22b4-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149126 http://www.mdpi.com/1996-1944/11/6/957 1 97dffed9-22b4-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149125 Biotehniška fakulteta 0 0 0