20.500.12556/RUL-131509 Novel spray dried glycerol 2-phosphate cross-linked chitosan microparticulate vaginal delivery system-development, characterization and cytotoxicity studies Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway. chitosan microparticles glycerol 2-phosphate spray drying mucoadhesiveness cytotoxicity hitosan mikrodelci citotoksičnost dostavni sistem glicerol 2-fosfat true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-29 08:10:11 2021-09-29 08:10:15 2022-09-10 04:12:07 0000-00-00 00:00:00 2016 0 0 22 str. iss. 10, art. 174 Vol. 14 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.1 1660-3397 10.3390/md14100174 4190065 RAZ_Szymańska_Emilia_2016.pdf RAZ_Szymańska_Emilia_2016.pdf 1 8A213E6543A2D2A37D20F3470BC7AD8D cb368f6bc5d95a5a80caec7ef80dc7e39132a718e694e31ce4568ac438e902c3 06fa9eb8-20ec-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148854 http://www.mdpi.com/1660-3397/14/10/174 1 a22e056f-20eb-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148853 Fakulteta za farmacijo 0 0 0