20.500.12556/RUL-131195 Exposure of free-ranging wild animals to zoonotic Leptospira interrogans sensu stricto in Slovenia A total of 249 serum samples from 13 wild animal species namely fallow deer (Dama dama, n = 1), roe deer (Capreolus capreolus, n = 80), red deer (Cervus elaphus, n = 22), chamois (Rupicapra rupicapra, n = 21), mouflon (Ovis musimon, n = 4), brown hare (Lepus europaeus, n = 2), nutria (Myocastor coypus, n = 1), red fox (Vulpes vulpes, n = 97), stone marten (Martes foina, n = 12), European badger (Meles meles, n = 2), golden jackal (Canis aureus, n = 2) Eurasian lynx (Lynx lynx, n = 2) and grey wolf (Canis lupus, n = 3) were analysed for the presence of antibodies against Leptospira interrogans sensu stricto. Serum samples were examined via the microscopic agglutination test for the presence of specific antibodies against Leptospira serovars Icterohaemorrhagiae, Bratislava, Pomona, Grippotyphosa, Hardjo, Sejroe, Australis, Autumnalis, Canicola, Saxkoebing and Tarassovi. Antibodies to at least one of the pathogenic serovars were detected in 77 (30.9%; CI = 25–37%) sera. The proportion of positive samples varied intraspecifically and was the biggest in large carnivores (lynx, wolf and jackal; 86%), followed by mezzo predators: stone marten (67%) and red fox (34%), and large herbivores: red deer (32%), roe deer (25%), alpine chamois (10%) and mouflon (0%). Out of the 77 positive samples, 42 samples (53.8%) had positive titres against a single serovar, while 35 (45.4%) samples had positive titres against two or more serovars. The most frequently detected antibodies were those against the serovar Icterohaemorrhagiae. The present study confirmed the presence of multiple pathogenic serovars in wildlife throughout Slovenia. It can be concluded that wild animals are reservoirs for at least some of the leptospiral serovars and are a potential source of leptospirosis for other wild and domestic animals, as well as for humans. wildlife Leptospira interrogans microscopic agglutination test serology Slovenia wild animals agglutination tests true false true Angleški jezik Ni določen Članek v reviji 2021-09-23 13:28:36 2021-09-23 13:28:36 2022-09-10 04:10:32 0000-00-00 00:00:00 2021 0 0 14 str. iss. 9, art. 2722 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616.9 2076-2615 10.3390/ani11092722 76969731 RAZ_Zele_Diana_2021.pdf RAZ_Zele_Diana_2021.pdf 1 3917A5B4C281030C1E2F810874EE6B46 8ab518436727af076e9606030f5b2b800422987ce7361ce999c0106c8ea299b3 b14b5ae6-1c62-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148505 https://www.mdpi.com/2076-2615/11/9/2722 0 1f56508a-1c61-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148467 Veterinarska fakulteta Biotehniška fakulteta 0 0 0