20.500.12556/RUL-131188 Supercritical carbon dioxide extraction of four medicinal mediterranean plants investigation of chemical composition and antioxidant activity With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest. green solvents chamomile yarrow St. John's wort curry plant square-wave voltammetry chromatography zelena topila kamilica rman šentjanževka curry voltametrija s kvadratnimi valovi kromatografija true false true Angleški jezik Ni določen Članek v reviji 2021-09-23 12:37:46 2021-09-23 12:37:46 2022-09-10 04:10:30 0000-00-00 00:00:00 2021 0 0 Str. 1-18 iss. 18 Vol. 26 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544 1420-3049 10.3390/ molecules26185697 77584387 https://www.mdpi.com/1420-3049/26/18/5697 1 056838ba-1c5a-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148458 RAZ_Cizmek_Lara_2021.pdf RAZ_Cizmek_Lara_2021.pdf 1 49D629FDC199CC47169A1507FD79F23F 9924ed31e48e5c8513de03fd86711a6cda68641a083e1cd394362a7df0d0e3e5 c2f3956e-1c5a-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148459 Zdravstvena fakulteta 0 0 0