20.500.12556/RUL-131188
Supercritical carbon dioxide extraction of four medicinal mediterranean plants
investigation of chemical composition and antioxidant activity
With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.
green solvents
chamomile
yarrow
St. John's wort
curry plant
square-wave voltammetry
chromatography
zelena topila
kamilica
rman
šentjanževka
curry
voltametrija s kvadratnimi valovi
kromatografija
true
false
true
Angleški jezik
Ni določen
Članek v reviji
2021-09-23 12:37:46
2021-09-23 12:37:46
2022-09-10 04:10:30
0000-00-00 00:00:00
2021
0
0
Str. 1-18
iss. 18
Vol. 26
2021
0000-00-00
Zaloznikova
Objavljeno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
543.544
1420-3049
10.3390/ molecules26185697
77584387
https://www.mdpi.com/1420-3049/26/18/5697
1
056838ba-1c5a-11ec-a523-00155dcfd717
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148458
RAZ_Cizmek_Lara_2021.pdf
RAZ_Cizmek_Lara_2021.pdf
1
49D629FDC199CC47169A1507FD79F23F
9924ed31e48e5c8513de03fd86711a6cda68641a083e1cd394362a7df0d0e3e5
c2f3956e-1c5a-11ec-a523-00155dcfd717
https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148459
Zdravstvena fakulteta
0
0
0