20.500.12556/RUL-131013 Rapid mechanistic evaluation and parameter estimation of putative inhibitors in a single-step progress-curve analysis the case of horse butyrylcholinesterase Highly efficient and rapid lead compound evaluation for estimation of inhibition parameters and type of inhibition is proposed. This is based on a single progress-curve measurement in the presence of each candidate compound, followed by the simultaneous analysis of all of these curves using the ENZO enzyme kinetics suite, which can be implemented as a web application. In the first step, all of the candidate ligands are tested as competitive inhibitors. Where the theoretical curves do not correspond to the experimental data, minimal additional measurements are added, with subsequent processing according to modified reaction mechanisms. enzymic mechanism inhibitors reactivators butyrylcholinestersae encimski mehanizem inhibitorji reaktivatorji true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-21 14:51:39 2021-09-21 14:51:43 2022-09-10 04:09:23 0000-00-00 00:00:00 2017 0 0 9 str. iss. 8, art. 1248 Vol. 22 Jul. 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1420-3049 10.3390/molecules22081248 34387929 RAZ_Stojan_Jure_2017.pdf RAZ_Stojan_Jure_2017.pdf 1 30E52D2393EB9CE70F2A3B29EF86DEE4 a466a7f44838b4908cd2afa6ba9cca3073e9c2ded34766687f6db26e672e88cf 911e89af-1ada-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148243 https://www.mdpi.com/1420-3049/22/8/1248 1 6808fb55-1ada-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148242 Medicinska fakulteta 0 0 0