20.500.12556/RUL-131011 In vitro innervation as an experimental model to study the expression and functions of acetylcholinesterase and agrin in human skeletal muscle Acetylcholinesterase (AChE) and agrin, a heparan-sulfate proteoglycan, reside in the basal lamina of the neuromuscular junction (NMJ) and play key roles in cholinergic transmission and synaptogenesis. Unlike most NMJ components, AChE and agrin are expressed in skeletal muscle and α-motor neurons. AChE and agrin are also expressed in various other types of cells, where they have important alternative functions that are not related to their classical roles in NMJ. In this review, we first focus on co-cultures of embryonic rat spinal cord explants with human skeletal muscle cells as an experimental model to study functional innervation in vitro. We describe how this heterologous rat-human model, which enables experimentation on highly developed contracting human myotubes, offers unique opportunities for AChE and agrin research. We then highlight innovative approaches that were used to address salient questions regarding expression and alternative functions of AChE and agrin in developing human skeletal muscle. Results obtained in co-cultures are compared with those obtained in other models in the context of general advances in the field of AChE and agrin neurobiology. acetylcholinesterase human muscle apoptosis in vitro innervation neuromuscular junction co-cultures agrin acetilholinesteraza človeška mišica apoptoza true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-21 13:43:36 2021-09-21 13:43:41 2022-09-10 04:09:22 0000-00-00 00:00:00 2017 0 0 30 str. iss. 9, art. 1418 Vol. 22 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-092 1420-3049 10.3390/molecules22091418 33379545 RAZ_Mis_Katarina_2017.pdf RAZ_Mis_Katarina_2017.pdf 1 A6500D3131D382265DBE2DBE0130CBBE 3523d809bd5e4c886b03a77c2abbb4cec0c4c59f10ed6aab3d1d3dcf81f31a88 b0a5ecf2-1ad2-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148239 http://www.mdpi.com/1420-3049/22/9/1418 1 e74918bb-1ad0-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=148238 Medicinska fakulteta 0 0 0