20.500.12556/RUL-130808 A novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended. porcine adenovirus cell culture urinary bladder transmission electron microscopy polymerase chain reaction microbiological screening prašičji adenovirus celične kulture sečni mehur true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-17 11:43:00 2021-09-17 11:43:04 2022-09-09 04:18:08 0000-00-00 00:00:00 2014 0 0 Str. 2505-2518 iss. 6 Vol. 6 Jun. 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1999-4915 10.3390/v6062505 31479257 RAZ_Dragin_Jerman_Urska_2014.pdf RAZ_Dragin_Jerman_Urska_2014.pdf 1 EC889F46087EAC5BDA4E4E59367FE124 0ce0b547267793913a856a7544f79e826eec595d54d5e1579bd744827c39258a f4185001-179b-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=147970 https://www.mdpi.com/1999-4915/6/6/2505 0 87696a55-179b-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=147969 Medicinska fakulteta 0 0 0