20.500.12556/RUL-130315 Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA The aim of the current study was to assess the antioxidative effects of the dietary supplementation of olive leaf extract (OLE) in different concentrations compared to those of vitamin E in piglets under conditions of dietary n-3 PUFA-induced oxidative stress. Forty-eight castrated male piglets (10.6 ± 0.99 kg) were fed the following experimental diets: Cont− (low-fat diet, no supplement), Cont+ (high linseed oil diet, no supplement), Vit-E (as Cont+, 105 IU vitamin E/day), OLE-1 (as Cont+, 3.84 mg hydroxytyrosol equivalents (HEQs)/day), OLE-2 (as Cont+, 38.4 mg HEQ/day), and OLE-3 (as Cont+, 96 mg HEQ/day). After 21 days of feeding, the experimental diets, blood and urine samples were collected to assess the extent of the oxidative stress. Results indicated that diet OLE-1 lowered the activity of gamma-glutamyl transferase, protected DNA (measured as DNA tail %) and altered urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG). Dietary vitamin E lowered the levels of urinary F$_2$-isoprostanes, as well as of plasma malondialdehyde and γ-tocopherol, but raised the plasmatic α-tocopherol and altered the level of urinary 8-OHdG. In conclusion, only minor positive effects of dietary OLE on the oxidative stress parameters were observed. Additionally, OLE did not show concentration dependence. hydroxytyrosol linseed oil olive polyphenols oxidative stress piglets vitamin E prašiči pujski prehrana živali maščobne kisline PUFA olive polifenoli vitamini vitamin E true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-13 14:22:25 2021-09-13 14:22:26 2023-06-02 14:10:44 0000-00-00 00:00:00 2019 0 0 11 str. iss. 4, art. 161 Vol. 9 2019 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 636.4.084/.087 2076-2615 10.3390/ani9040161 4204936 RAZ_Leskovec_Jakob_2019.pdf RAZ_Leskovec_Jakob_2019.pdf 1 E6F3287579466D58520AA26961CC58A8 63c620b1ede8de611e9280bdb0cd0ee5be295a1cd282ec5c618347250796056c d76985c2-31ab-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=149876 https://www.mdpi.com/2076-2615/9/4/161 1 0109d8c9-148d-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=147400 Biotehniška fakulteta Veterinarska fakulteta 0 0 0