20.500.12556/RUL-129747 Določevanje vezavnih mest desnosučnih aminokislin v peptidih in proteinih Determination of bonding sites of D-amino acids in peptides and proteins Desnosučne aminokisline so v majhnih količinah prisotne tako v sintetičnih kot tudi v nekaterih naravnih peptidih in proteinih. Določevanje vezavnih mest desnosučnih aminokislin je pomembno za podrobno analizo in razumevanje teh peptidov in proteinov. V diplomskem delu sem predstavil metode za analizo proteinov, ki vsebujejo desnosučne aminokisline. Vse metode za določevanje vezavnih mest desnosučnih aminokislin temeljijo na masni spektrometriji, ki je ključna tehnika za analizo zgradbe proteinov. Cilj eksperimentalnega dela je bil razviti metodo za ločbo proteinov, ki vsebujejo desnosučne aminokisline. Metodo sem razvijal na dveh tandemskih masnih spektrometrih, ki sta bila sklopljena s tekočinskima kromatografoma. D-amino acids are found in small quantities both in natural and synthetic peptides and proteins. Determination of their bonding sites is important for detailed analysis and understanding of these peptides and proteins. In my diploma thesis I presented methods for analysis of proteins that consist of D-amino acids. All methods for determination of bonding sites of D-amino acids are based on mass spectrometry, which is the key technique for analysing the structure of proteins. The goal of the experimental part was to develop a method to separate proteins consisting of D-amino acids. The method was developed with two tandem mass spectrometers, which were coupled with liquid chromatography instruments. D-aminokisline peptidi proteini stereoizomeri masna spektrometrija D-amino acids peptides proteins stereoisomers mass spectrometry true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2021-09-07 18:05:06 2021-09-07 18:05:08 2022-09-09 04:13:05 0000-00-00 00:00:00 2021 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 18461 79533059 Puhar_Gregor_-_Dolocevanje_vezavnih_mest_desnosucnih_aminokislin_v_peptidih_in_proteinih.pdf Puhar_Gregor_-_Dolocevanje_vezavnih_mest_desnosucnih_aminokislin_v_peptidih_in_proteinih.pdf 1 B3CF3723797BACD672EEE549045CC220 f4995ddfb037dc9e2f06ea257338aa2d8241089c2cdc85cb08246b6fc854f5f4 2102d996-0ff5-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146774 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0