20.500.12556/RUL-129734 Mehanizmi sproščanja proteinov iz hidrogelov Mechanisms of protein release from hydrogels S pojavom različnih proteinskih terapevtikov se je na področju farmacije in tkivnega inženirstva pojavila potreba po razvoju novih sistemov, ki bi zagotavljali dostavo proteinov do izbranega tarčnega tkiva in njihovo tamkajšnje kontrolirano sproščanje. Za ta namen so se kot optimalni izkazali hidrogeli. Odlikujejo jih visoka vsebnost vode in v primeru, da hidrogelno mrežo tvorijo biopolimeri, tudi biokompatibilnost in nestrupenost. Vodno okolje znotraj hidrogelov dobro simulira fiziološko okolje ter omogoča ohranjanje naravne strukture proteinov. V hidrogelih naložene molekule proteina se lahko v okoliško tkivo enakomerno sproščajo skozi daljše časovno obdobje. V splošnem lahko sproščanje uravnavajo različni mehanizmi. Na kratko so predstavljene ključne strukturne lastnosti hidrogelov in proteinov, ki v dobršni meri determinirajo način, na katerega bo sproščanje potekalo. Navedeni so kvantitativni kriteriji, na podlagi katerih je moč oceniti, kateri mehanizem bo odločilno vplival na profil izločanja proteina. Posamezni mehanizmi so na kratko opisani, posebna pozornost pa je posvečena trenutno zelo aktualnim afinitetnim dostavnim sistemom. Po vzoru iz literature je prikazan razvoj matematičnega modela za sproščanje proteinov iz afinitetnega hidrogela. With the advent of various protein therapeutics in the field of pharmacy and tissue engineering, a need to develop new systems that would ensure the delivery of proteins to the selected target tissue and their controlled release out there has emerged. For this purpose hydrogels proved to be the optimal choice. They are distinguished by a high water content and, in case the hydrogel network is made of biopolymers, also by biocompatibility and non-toxicity. The aqueous environment within hydrogels efficiently simulates the physiological environment and preserves the native structure of proteins. Protein molecules loaded inside the hydrogel can be released into the surrounding tissue continuously over a long period of time. In general protein release can be modulated by different mechanisms. The key structural features of hydrogels and proteins which to a great extent determine the way in which release takes place are presented briefly. Quantitative criteria to determine which mechanism will have a decisive impact on protein release profile are stated. Each individual mechanism is described in brief and particular attention is paid to affinity-based delivery systems nowadays being of special interest. Based on the literature, the development of a mathematical model for protein release from an affinity-based hydrogel is presented. protein hidrogel mehanizem sproščanja profil izločanja protein hydrogel release mechanism release profile true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2021-09-07 13:45:00 2021-09-07 13:45:04 2022-09-09 04:13:01 0000-00-00 00:00:00 2021 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 16482 79296259 Golobic_Jurij_-_Mehanizmi_sproscanja_proteinov_iz_hidrogelov.pdf Golobic_Jurij_-_Mehanizmi_sproscanja_proteinov_iz_hidrogelov.pdf 1 E3B94355B570BAC8198EA0E42FAE5FFB d28e69720220b40278adecdb633a8d23fa83e3f9386eb7c4604b873940305d51 cc5aa3d8-0fd0-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146760 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0