20.500.12556/RUL-129641 Detekcija sproščenega heksanala s plinsko kromatografijo Detection of released hexanal with gas chromatography Heksanal (oz. heksanaladehid) je alkil aldehid, ki se uporablja v industriji arom za živila in produkte s sadnim okusom in vonjem. Je hlapna spojina s protimikrobnim delovanjem, zato se ga pogosto uporablja za zaviranje zorenja sadja in zelenjave. Namen diplomskega dela je določiti količino sproščenega heksanala s plinsko kromatografijo. Kot detektor smo uporabili masni spektrometer, ki ponuja visoko selektivnost in občutljivost. GC-MS pogoje smo optimirali tako, da smo dobili dovolj kvalitetne spektre posameznih spojin in s tem primerno ločitev komponent. Analizno metodo smo potrdili in pokazali njeno linearnost, natančnost in ponovljivost. Analizirali smo tudi tri realne vzorce encimov, ki sproščajo heksanal. Največ heksanala se je sprostilo pri mešanici encimov lipaze in lipoksidaze, v vzorcu same lipoksidaze pa nismo zasledili večjih količin sproščenega heksanala, tudi po daljšem časovnem obdobju. Hexanal (or hexanaldehyde) is an alkylaldehyde used in the flavor industry for foods and products with a fruity taste and aroma. It is a naturally volatile compound with antimicrobial activity, which is why it is often used to inhibit the ripening of fruits and vegetables. The aim of this work is to determine the amount of hexanal released by gas chromatography. Te detector used is a mass spectrometer, which offers high selectivity and sensitivity. GC-MS conditions were optimized to achieve sufficient quality of the spectra of the individual compounds and thus adequate separation of the components. The analytical method was validated to demonstrate its linearity, accuracy and repeatability. We also analyzed three real samples of hexanal releasing enzymes. Most of the hexanal was released by the mixture of lipase and lipoxidase enzymes, and no large amounts of released hexanal were detected in the lipoxidase sampel itself, even after a long time. heksanal GC izbira topila headspace analiza hexanal GC solvent headspace analysis true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2021-09-06 12:39:34 2021-09-06 12:39:37 2022-09-08 04:09:47 0000-00-00 00:00:00 2021 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 18662 76826115 Kuret_Tajda_-_Merjenje_sproscenega_heksanala_iz_materiala_z_visoko_zmogljivo_tekocinsko_kromatog.pdf Kuret_Tajda_-_Merjenje_sproscenega_heksanala_iz_materiala_z_visoko_zmogljivo_tekocinsko_kromatog.pdf 1 E9A54C06C33FFEEB627125B3F34314CA 78651b7dfe668de04e2b6a66a6a3665d7385602a090dabfabe70de38e4c2e2e8 7d7a973c-0efe-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146656 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0