20.500.12556/RUL-129636 Method for controlled tissue theranostics using a single tunable laser source Tissue diseases and related disorders need to be first recognized using diagnostic methods and then later treated by therapeutic methods–a joint procedure called theranostics. One of the main challenges in the field of retinal therapies remains in the success of the treatment, typically improving the local metabolism, by sparing the surrounding tissue and with the immediate information of the laser effect. In our study, we present a concept for real-time controlled tissue theranostics on a proof-of-concept study capable of using a single tunable ps laser source (in terms of irradiance, fluence, and repetition rate), done on ex-vivo human retinal pigment epithelium. We have found autofluorescence intensity and lifetime imaging diagnostics very promising for the recognition and quantification of laser effects ranging from selective non-destructive molecular tissue modification to complete tissue ablation. The main novelty of our work presents the developed algorithm for optimized theranostics based on the model function used to quantify laser-induced tissue changes through the diagnostics descriptors, fluorescence lifetime and fluorescence intensity parameters. This approach, together with the operation of the single adaptable laser source, can serve as a new theranostics method in personalized medicine in the future not only limited to treat retinal diseases. adaptable fiber lasers retinal tissue theranostics multimodal imaging fluorescence lifetime imaging prilagodljivi vlakenski laserji tkivo mrežnice teranostika multimodalno slikanje življenjski čas fluorescence true false true Angleški jezik Slovenski jezik V članku navedeno: "© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement"; s povezavo https://www.osapublishing.org/library/license_v1.cfm. (6. 9. 2021) Članek v reviji 2021-09-06 12:30:28 2021-09-06 12:30:28 2023-05-29 10:37:19 0000-00-00 00:00:00 2021 0 0 Str. 5881-5893 no. 9, art. 428467 Vol. 12 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 535 2156-7085 10.1364/BOE.428467 75069699 RAZ_Podlipec_Rok_2021.pdf RAZ_Podlipec_Rok_2021.pdf 1 82351FD6F9D34ABD11E30746EBFABA4B 505d7789e20c86eb81fdb6be9b4582a3e98236adbec9bd14e1644d5dbb0895ea 3225390d-0eff-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146674 https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-12-9-5881&id=458320 0 40165c82-0eff-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146675 Fakulteta za strojništvo 0 0 0