20.500.12556/RUL-129622 Active protein aggregates produced in Escherichia coli Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins can be prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing. E. coli active protein aggregates inclusion bodies IBs recombinant proteins biotechnology Escherichia coli rekombinantne beljakovine inkluzijska telesca biotehnologija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-09-06 08:43:58 2021-09-06 08:44:01 2023-05-26 03:41:39 0000-00-00 00:00:00 2011 0 0 Str. 8275-8287 iss. 11 Vol. 12 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1422-0067 10.3390/ijms12118275 29115609 RAZ_Peternel_Spela_2011.pdf RAZ_Peternel_Spela_2011.pdf 1 6CC6BC7F3BC0186167D42D4CB444C960 7f8b15f0464f0678bb5998df78fc2f9a6ae8e71013d153585d93edcdd5a65bf2 efc51b20-0edd-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146632 https://www.mdpi.com/1422-0067/12/11/8275 0 d8f9deee-0edd-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146631 Medicinska fakulteta 0 0 0