20.500.12556/RUL-129250 Magnetic resonance imaging for rapid screening for the nephrotoxic and hepatotoxic effects of microcystins In vivo visualization of kidney and liver damage by Magnetic Resonance Imaging (MRI) may offer an advantage when there is a need for a simple, non-invasive and rapid method for screening of the effects of potential nephrotoxic and hepatotoxic substances in chronic experiments. Here, we used MRI for monitoring chronic intoxication with microcystins (MCs) in rat. Male adult Wistar rats were treated every other day for eight months, either with MC-LR (10 μg/kg i.p.) or MC-YR (10 μg/kg i.p.). Control groups were treated with vehicle solutions. T$_1$-weighted MR-images were acquired before and at the end of the eight months experimental period. Kidney injury induced by the MCs presented with the increased intensity of T$_1$-weighted MR-signal of the kidneys and liver as compared to these organs from the control animals treated for eight months, either with the vehicle solution or with saline. The intensification of the T$_1$-weighted MR-signal correlated with the increased volume density of heavily injured tubuli (R$^2$ = 0.77), with heavily damaged glomeruli (R$^2$ = 0.84) and with volume density of connective tissue (R$^2$ = 0.72). The changes in the MR signal intensity probably reflect the presence of an abundant proteinaceous material within the dilated nephrons and proliferation of the connective tissue. T$_1$-weighted MRI-is a valuable method for the in vivo screening of kidney and liver damage in rat models of intoxication with hepatotoxic and nephrotoxic agents, such as microcystins. microcystin chronic toxicity magnetic resonance imaging kidney liver nephrotoxic mikrocistin kronična toksičnost slikanje z magnetno resonanco true false true Angleški jezik Slovenski jezik Članek v reviji 2021-08-31 11:43:32 2021-08-31 11:43:36 2022-09-08 04:07:30 0000-00-00 00:00:00 2013 0 0 Str. 2785-2798 iss. 8 Vol. 11 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.1 1660-3397 10.3390/md11082785 30744025 RAZ_Milutinovic_Zivin_Aleksandra_2013.pdf RAZ_Milutinovic_Zivin_Aleksandra_2013.pdf 1 6A9E89A415630321EDE15B896BE958B0 ab317da7eb483274080b258d4fc4d51ad27f9a3d165c9319e2e9bdc7fa5a1f55 f21696d7-0a3f-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146217 http://www.mdpi.com/1660-3397/11/8/2785 1 af7fe017-0a3f-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146215 Medicinska fakulteta 0 0 0