20.500.12556/RUL-128834 Simultaneous visualization of myosin heavy chain isoforms in single muscle sections We developed a staining protocol that enables simultaneous visualization of myosin heavy chain (MHC) pure and hybrid muscle fiber types in rat skeletal muscle. Up to eight differ-ent muscle fiber types can be visualized in a single section of the rat extensor digitorum longus muscle, which contains all four adult MHC isoforms and shows plasticity during the denervation-reinnervation process. Triple immunofluores-cent staining of MHC-1, MHC-2a and MHC-2b with primary antibodies BA-D5 (isotype IgG2b), SC-71 (isotype IgG1) and BF-F3 (isotype IgM) and with three fluorophore-labeled iso-type-specific secondary antibodies displays different mus-cle fiber types in a merged image of red, green and blue channels, each in its own color. Immunoperoxidase staining with primary antibody 6H1 directed against MHC-2x can be additionally applied on the same tissue section to facilitate the identification of muscle fibers containing MHC-2x. Triple staining can also be used in combination with other staining procedures to derive more information about the number of capillaries or the oxidative potential of muscle fiber types. Simultaneous visualization of multiple fiber types in a single merged image enables economical use of muscle samples and provides simple and rapid identification of all fiber types that are present in rat limb muscles. histochemistry immunofluorescent staining muscle fiber types histokemija imunofluorescenčni madeži vrste mišičnih vlaken true false true Angleški jezik Slovenski jezik Članek v reviji 2021-08-05 11:47:42 2021-08-05 11:47:44 2022-09-07 04:17:23 0000-00-00 00:00:00 2013 0 0 Str. 312-321 iss. 4 Vol. 197 2013 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-092 1422-6405 10.1159/000345424 30372825 RAZ_Ribaric_Samo_2013.pdf RAZ_Ribaric_Samo_2013.pdf 1 F01CD86ECCF8DE071141A011757B8745 82cd27c32ed8f4ba3b6c98c87616248d59feec7563e30d7b20778997ba191504 4ef906cd-f5d2-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145731 https://www.karger.com/Article/Abstract/345424 0 574d8091-f5d2-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145732 Medicinska fakulteta 0 0 0