20.500.12556/RUL-128831 Methods for studying endocytotic pathways of herpesvirus encoded G protein-coupled receptors Endocytosis is a fundamental process involved in trafficking of various extracellular and transmembrane molecules from the cell surface to its interior. This enables cells to communicate and respond to external environments, maintain cellular homeostasis, and transduce signals. G protein-coupled receptors (GPCRs) constitute a family of receptors with seven transmembrane alpha-helical domains (7TM receptors) expressed at the cell surface, where they regulate physiological and pathological cellular processes. Several herpesviruses encode receptors (vGPCRs) which benefits the virus by avoiding host immune surveillance, supporting viral dissemination, and thereby establishing widespread and lifelong infection, processes where receptor signaling and/or endocytosis seem central. vGPCRs are rising as potential drug targets as exemplified by the cytomegalovirus-encoded receptor US28, where its constitutive internalization has been exploited for selective drug delivery in virus infected cells. Therefore, studying GPCR trafficking is of great importance. This review provides an overview of the current knowledge of endocytic and cell localization properties of vGPCRs and methodological approaches used for studying receptor internalization. Using such novel approaches, we show constitutive internalization of the BILF1 receptor from human and porcine γ-1 herpesviruses and present motifs from the eukaryotic linear motif (ELM) resources with importance for vGPCR endocytosis. endocytosis G-protein coupled receptors herpesvirus methods Herpesviridae true false true Angleški jezik Ni določen Članek v reviji 2021-08-04 13:13:39 2021-08-04 13:13:39 2022-09-07 04:17:22 0000-00-00 00:00:00 2020 0 0 23 str. iss. 23, art. 5710 Vol. 25 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616 1420-3049 10.3390/molecules25235710 40808963 RAZ_Mavri_Masa_2020.pdf RAZ_Mavri_Masa_2020.pdf 1 77B37009C29D1BAE3BD841236EBCD015 bb10934c2f5ce73487164d292608a7676bb214caeea056ecfa1491b8d2b631f1 435bc819-85ba-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153559 https://www.mdpi.com/1420-3049/25/23/5710 1 d68b579f-f514-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145721 Veterinarska fakulteta 0 0 0