20.500.12556/RUL-128788 Epithelial-mesenchymal transition-related microRNAs and their target genes in colorectal cancerogenesis MicroRNAs of the miR-200 family have been shown experimentally to regulate epithelial-mesenchymal transition (EMT). Although EMT is the postulated mechanism of development and progression of colorectal cancer (CRC), there are still limited and controversial data on expression of miR-200 family and their target genes during CRC cancerogenesis. Our study included formalin-fixed paraffin-embedded biopsy samples of 40 patients (10 adenomas and 30 cases of CRC with corresponding normal mucosa). Expression of miR-141, miR-200a/b/c and miR-429 and their target genes (CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2 and ZEB2) was analysed using quantitative real-time PCR. Expression of E-cadherin was analysed using immunohistochemistry. All miRNAs were down-regulated and their target genes showed the opposite expression in CRC compared to adenoma. Down-regulation of the miR-200 family at the invasive front in comparison to the central part of tumour was observed as well as a correlation of expression of miR-200b, CDKN1B, ONECUT2 and ZEB2 expression to nodal metastases. Expression of the miR-200 family and SOX2 also correlated with E-cadherin staining. These results suggest that the miR-200 family and their target genes contribute to progression of adenoma to CRC, invasive properties and development of metastases. Our results strongly support the postulated hypotheses of partial EMT and intra-tumour heterogeneity during CRC cancerogenesis. colorectal adenoma colorectal carcinoma metastases intra-tumour heterogeneity epithelial-mesenchymal transition miR-200 family target genes kolorektalni adenom kolorektalni karcinom metastaze intra-tumorska heterogenost epitelno-mezenhimska tranzicija družina miR-200 tarčni geni true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-29 15:11:07 2021-07-29 15:11:09 2022-09-07 04:17:12 0000-00-00 00:00:00 2019 0 0 21 str. iss. 10, art. 1603 Vol. 8 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2077-0383 10.3390/jcm8101603 34547929 RAZ_Rankovic_Branislava_2019.pdf RAZ_Rankovic_Branislava_2019.pdf 1 CF7D808F8E575761D10E16F4945EC8E5 b53a053290cd9ac9e581bd2f9e24621e8e555015a09b68df2207fec2432363e3 4676e418-f06e-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145669 https://www.mdpi.com/2077-0383/8/10/1603 0 68829314-f06e-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145670 Medicinska fakulteta 0 0 0