20.500.12556/RUL-128774 Disinfection of infected artificial dental periapical lesions with diode laser an in vitro study Background: Periapical lesions are primarily caused by infections in the root canals. The objective of this study was to assess the antibacterial effectiveness of diode laser during root canal treatment in artificial models of infected periapical lesions. Material and methods: One hundred twenty-two extracted premolar single-rooted teeth were inserted into methyl methacrylate artificial models of periapical lesions, and bacterial solutions of Enterococcus faecalis (ATCC 29212) and Streptococcus mitis (ATCC 49456) were then applied to the models. The respective diameters of lesions in the artificial models represented 3 different subgroups based on lesion size. The laser protocol used for endodontic disinfection had a power output of 1.5 W and a wavelength of 810 nm. The impact on cell viability was evaluated by flow cytometry. Results: Disinfection with laser did not differ between microorganisms (P=0.137), and laser irradiation with a longer duration had better disinfecting action for both microorganisms (P<0.001). Compared with larger lesions, smaller lesions had a higher percentage of dead cells for both microorganisms (P<0.001). The percentage of dead cells in the treatment groups was significantly higher than in the control group (P<0.001). Conclusions: Laser treatment had a poor, almost negligible effect on elimination of bacterial cells in large periapical lesions. Application of a laser might serve as an adjuvant method to standard irrigation with sodium hypochlorite. Enterococcus faecalis lasers periapical diseases streptococcus mitis root canal therapy Enterococcus Faecalis laserji periapikalne bolezni streptococcus mitis koreninsko zdravljenje true false true Angleški jezik Ni določen Neznano 2021-07-28 13:06:00 2021-07-28 13:06:08 2022-09-07 04:17:07 0000-00-00 00:00:00 2021 0 0 Str. e932492-1 - e932492-7 Vol. 27 Jul. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-03-31 2021-06-08 2021-07-27 616.31:614.48 2325-4416 71706115 https://basic.medscimonit.com/abstract/index/idArt/932492 1 a638533f-ef93-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145643 RAZ_Bytyqi_Agron_2021.pdf RAZ_Bytyqi_Agron_2021.pdf 1 3DD28CB85FEED94D1926A7B2E2738481 11ffee3d4d529e664979ce5925562d0d23bffeb36c9fc345fc9f6d13b451c523 0b7ab5bc-ef94-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145644 Zdravstvena fakulteta 0 0 0