20.500.12556/RUL-128743 ZIKV strains differentially affect survival of human fetal astrocytes versus neurons and traffic of ZIKV-laden endocytotic compartments Malformations of the fetal CNS, known as microcephaly, have been linked to Zika virus (ZIKV) infection. Here, the responses of mammalian and mosquito cell lines, in addition to primary human fetal astrocytes and neurons were studied following infection by ZIKV strains Brazil 2016 (ZIKV-BR), French Polynesia 2013 (ZIKV-FP), and Uganda #976 1947 (ZIKV-UG). Viral production, cell viability, infectivity rate, and mobility of endocytotic ZIKV-laden vesicles were compared. All cell types (SK-N-SH, Vero E6, C6/36, human fetal astrocytes and human fetal neurons) released productive virus. Among primary cells, astrocytes were more susceptible to ZIKV infection than neurons, released more progeny virus and tolerated higher virus loads than neurons. In general, the infection rate of ZIKV-UG strain was the highest. All ZIKV strains elicited differences in trafficking of ZIKV-laden endocytotic vesicles in the majority of cell types, including astrocytes and neurons, except in mosquito cells, where ZIKV infection failed to induce cell death. These results represent a thorough screening of cell viability, infection and production of three ZIKV strains in five different cell types and demonstrate that ZIKV affects vesicle mobility in all but mosquito cells. microcephaly human fetal astrocytes human fetal neurons mikrocefalija humani fetalni astrociti človeški fetalni nevroni true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-27 10:30:10 2021-07-27 10:30:14 2022-09-07 04:16:57 0000-00-00 00:00:00 2019 0 0 14 str. art. 8069 Vol. 9 May 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-092 2045-2322 10.1038/s41598-019-44559-8 34336729 RAZ_Jorgacevski_Jernej_2019.pdf RAZ_Jorgacevski_Jernej_2019.pdf 1 6FA8E43AFE920C4F97D484E9A2CE25A8 24daed4e7390960456ea53452b033e282bf44f697ba9391fa9b96ea1eb25c6c1 b350224c-eeb4-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145586 https://www.nature.com/articles/s41598-019-44559-8 0 d2d8f84c-eeb4-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145587 Medicinska fakulteta 0 0 0