20.500.12556/RUL-128695 Inflammation and its association with oxidative stress in dogs with heart failure Background: Inflammation and oxidative stress can contribute to the development and progression of heart failure. This study aimed to investigate the association between inflammatory and oxidative stress markers in dogs with congestive heart failure (CHF). Associations between the disease severity marker N-terminal pro-B-type natriuretic peptide (NT-proBNP) and markers of inflammation and oxidative stress were also determined. Results: Thirty-seven dogs with cardiovascular diseases (dilated cardiomyopathy, DCM (16 dogs), myxomatous mitral valve disease, MMVD (21 dogs)) and ten healthy dogs were included in this prospective study. The patients were further divided into groups with (26) and without CHF (11). We found a significantly higher serum concentration of C-reactive protein (P = 0.012), white blood cell (P = 0.001), neutrophil (P = 0.001) and monocyte counts (P = 0.001) in patients with CHF compared to control dogs. The concentration of tumor necrosis factor-alpha (TNF-α) was significantly higher in patients with CHF compared to patients without CHF (P = 0.030). No significant difference was found in most of the measured parameters between MMVD and DCM patients, except for glutathione peroxidase (GPX) and NT-proBNP. In patients with CHF, TNF-α correlated positively with malondialdehyde (P = 0.014, r = 0.474) and negatively with GPX (P = 0.026, r = − 0.453), and interleukin-6 correlated negatively with GPX (P = 0.046, r = − 0.412). NT-proBNP correlated positively with malondialdehyde (P = 0.011, r = 0.493). In patients without CHF none of the inflammatory and oxidative stress markers correlated significantly. Furthermore, in the group of all cardiac patients, GPX activity significantly negatively correlated with NT-proBNP (P = 0.050, r = − 0.339) and several markers of inflammation, including TNF-α (P = 0.010, r = − 0.436), interleukin-6 (P = 0.026, r = − 0.382), white blood cell (P = 0.032, r = − 0.369), neutrophil (P = 0.027, r = − 0.379) and monocyte counts (P = 0.024, r = − 0.386). Conclusion: Inflammatory and oxidative stress markers are linked in canine CHF patients, but not in patients without CHF. These results suggest complex cross communication between the two biological pathways in advanced stages of CHF. canine congestive heart failure C–reactive protein interleukin–6 malondialdehyde tumour necrosis factor–alpha white blood cell count heart failure leukocyte count dogs true false true Angleški jezik Ni določen Članek v reviji 2021-07-23 11:25:59 2021-07-23 11:26:00 2023-03-24 10:49:26 0000-00-00 00:00:00 2021 0 0 10 str. art. 176 Vol. 17 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.7.09:616 1746-6148 10.1186/s12917-021-02878-X 61194499 RAZ_Nemec_Svete_Alenka_2021.pdf RAZ_Nemec_Svete_Alenka_2021.pdf 1 14069A18B75AA6A5A481D0D1E30C194D 6000e75f060fdb4bb0553e13e45cb913455e693de19e14b41010a9a0208bc1e8 d4cfb9f1-eb97-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145520 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-021-02878-x 0 0f041cd1-ca27-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167521 Veterinarska fakulteta Biotehniška fakulteta 0 0 0