20.500.12556/RUL-128499 Vloga naravnih spojin rastlinskega izvora v avtofagiji The role of natural plant Compounds in autophagy Eden izmed ključnih, evolucijsko ohranjenih, preživetvenih procesov je avtofagija, katero lahko induciramo tudi s pomočjo naravnih spojin rastlinskega izvora. Le-te najpogosteje izvirajo iz rastlin in so del tradicionalne kitajske medicine. Najbolj preučene so resveratrol, DMC, berberin in celastrol, ki delujejo na zelo različne načine. Resveratrol inducira avtofagijo preko mTOR kompleksa ter razgradnje klasičnega receptorja avtofagije p62, DMC deluje odvisno od Atg proteinov, celastrol preko inhibicije signalne poti Akt/mTOR in povečanja izražanja avtofagosomov in p62, berberin pa preko hranilnega senzorja SIRT1. Vsem štirim naravnim spojinam je skupno, da učinkovito inducirajo avtofagijo tarčnih celic in ne poškodujejo sosednjih, zdravih celic. Tako predstavljajo velik potencial v medicini za zdravljenje rakavih in drugih obolenj, zato je bil naš namen pregled literature o vlogi naravnih spojin rastlinskega izvora v avtofagiji ter pregled že izvedenih študij o potencialni uporabi le-teh pri kemoterapiji. Zaradi dvoličnosti avtofagije, ki je lahko zaviralec, a tudi spodbujevalec raka, je zelo pomembno poznavanje stadija tumorja, makrookolja ter ustrezne kombinacije med avtofagija-inducirajočo spojino in kemterapevtikom. Kot najboljše naravne spojine so se izkazali resveratrol, celastrol in DMC, ki inducirajo avtofagijo v rakavih celicah ter zmanjšajo citotoksičen učinek kemoterapevtika na okoljske zdrave celice. Naravne spojine same po sebi sicer že inducirajo avtofagijo, a je njihov učinek na omejitev rasti rakavih celic boljši takrat, ko so uporabljene v kombinaciji z ustreznim kemoterapevtikom. One of the crucial evolutionarily preserved survival processes is autophagy, which can also be induced with the help of natural compounds of plant origin. These most commonly originate from plants and are part of Chinese traditional medicine. The most studied compounds are resveratrol, DMC, berberine and ceastrol, which work in very different ways. Resveratrol induces autophagy via the mTOR complex and degradation of the classical p62 autophagy receptor, DMC works dependent of Atg proteins, celastrol inhibits Akt/mTOR signaling pathway and increases expression of autophagosomes and p62 proteins and berberin works via the SIRT1 nutrient sensor. All four natural compounds have in common that they effectively induce target cell autophagy and do not damage neighboring, healthy cells. Thus, they represent a great potential in medicine for the treatment of cancer and other diseases, so our purpose was to review the literature on natural compounds of plant origin and review already conducted studies on their potential use in chemotherapy. Due to the hypocrisy of autophagy that can be inhibits or promotes cancer, it is very important to know the stage of the tumor, the microenvironment and the appropriate combination of cancer inhibitors based on autophagy and chemotherapeutics. Resveratrol, celastrol and DMC have been shown to be the best natural compounds, inducing autophagy in tumor cells and preventing the cytotoxic effect of the chemotherapeutic on healthy cells. It is known that natural compounds already induce autophagy themselves, but have a better effect on limiting the growth of cancer cells when used in a combination with appropriate chemotherapeutics. avtofagija naravne spojine rastlinske snovi celastrol resveratrol DMC berberin autophagy natural compounds celastrol resveratrol DMC berberin plant compounds true false false [E. Molan] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2021-07-15 10:56:19 2021-07-15 10:56:31 2022-09-07 04:15:33 0000-00-00 00:00:00 2021 0 Ljubljana 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 606:61:615.322(043.2) 191455 70079491 15366.pdf 15366.pdf 1 21D688BEDF1639E06907102E3D4F667C a7e68aac3ffa480cbd2fb4c85d44865e183bd5b0f2f6dbd05071d9c61517f1ff 67416e4b-e54a-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145253 Biotehniška fakulteta 0 0 0